TRP Channels Methods and Protocols
This book aims to provide a guide to researchers on how to work with Transient Receptor Potential (TRP) channels, describing current methodologies and protocols, and highlighting the challenges ahead that will require the development of new technologies.
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Antonio Ferrer-Montiel Tim Hucho Editors
TRP Channels Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
TRP Channels Methods and Protocols
Edited by
Antonio Ferrer-Montiel Instituto de Biología Molecular y Celular, Universidad Miguel Hernández, Elche, Alicante, Spain
Tim Hucho Experimental Anesthesiology and Pain Research, Department of Anesthesiology and Intensive Care Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany
Editors Antonio Ferrer-Montiel Instituto de Biologı´a Molecular y Celular Universidad Miguel Herna´ndez Elche, Alicante, Spain
Tim Hucho Experimental Anesthesiology and Pain Research Department of Anesthesiology and Intensive Care Medicine University Hospital Cologne University of Cologne Cologne, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9445-8 ISBN 978-1-4939-9446-5 (eBook) https://doi.org/10.1007/978-1-4939-9446-5 © Springer Science+Business Media, LLC, part of Springer Nature 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Transient receptor potential (TRP) channels have been the focus of research for many decades with still ever-increasing interest. They form a family of currently up to 28 different channel members grouped in 7 families, namely TRPC, TRPM, TRPV, TRPA, TRPN, TRPML, and TRPP, with an additional TRPY family identified in yeast. They show a surprising variety of molecular mechanisms regulating their opening and are involved i
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