Twenty-two novel microsatellite loci for lumpfish ( Cyclopterus lumpus )
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TECHNICAL NOTE
Twenty-two novel microsatellite loci for lumpfish (Cyclopterus lumpus) Sigurlaug Skirnisdottir • Gudbjorg Olafsdottir • Kristinn Olafsson Thomas Jendrossek • Hywel ap Dafydd Lloyd • Sarah Helyar • Christophe Pampoulie • Anna K. Danielsdottir • Jacob M. Kasper
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Received: 17 August 2012 / Accepted: 22 August 2012 / Published online: 23 September 2012 Ó Springer Science+Business Media B.V. 2012
Abstract Lumpfish (Cyclopterus lumpus L. 1758) are widely distributed on both sides of the North Atlantic. They are a commercially important species, but stock size estimates have declined since the mid-1980s in Canada, Norway and Iceland. Little is known about the biology of this species, in particular the breeding migrations and population structure which are fundamental for effective management. This paper describes the development and characterization of twenty-two polymorphic microsatellite loci using next generation sequencing. The number of alleles per locus ranged from 3 to 27 in two geographically distant North Atlantic populations, with observed and expected heterozygosities ranging between 0.0625–0.979 and 0.0618–0.946, respectively. These loci are an important resource that will allow assessment of the population genetic structure of this species, and contribute to its appropriate management. Keywords Lumpfish Microsatellite Genotyping Next generation sequencing
Lumpfish (Cyclopterus lumpus L. 1758) are widely distributed throughout the North Atlantic Ocean. Adult fish move considerable distances between the offshore feeding areas into shallow coastal water during the spawning season
S. Skirnisdottir (&) G. Olafsdottir K. Olafsson T. Jendrossek H. a. D. Lloyd S. Helyar A. K. Danielsdottir Matı´s Ltd., Vı´nlandsleið 12, 113 Reykjavı´k, Iceland e-mail: [email protected] C. Pampoulie J. M. Kasper Marine Research Institute, Sku´lagata 4, 101 Reykjavı´k, Iceland J. M. Kasper BioPol, Einbu´astı´gur 2, 545 Skagastro¨nd, Iceland
(March–August) and then return to deeper and colder water. Females lay their eggs on rocky shores in seaweed beds and the males nurture the eggs until they hatch (Davenport 1985; Ingolfsson et al. 2002; Schopka 1974; Thorsteinsson 1981). Juveniles emerge from egg clutches in the spring and spend their first year among weed clumps in bays and fjords until they move offshore (Ingolfsson 2000; Ingolfsson et al. 2002) where they have been caught in both pelagic fishing gear (Blacker 1983; Holst 1993) and benthic trawls (Hoenig and Hewitt 2005). It is not known if the populations are subdivided, however, there are indications that there is some degree of philopatry to spawning sites (Schopka 1974; Thorsteinsson 1981), suggesting that stock structure could be maintained. Lumpfish are commercially important in Canada, Iceland, Greenland and Norway, where the females are targeted for their roe, which is harvested as a caviar substitute. Despite its economic importance, little information is available on the biology of this species, and in particular its stock size and s
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