Uptake and imaging of glycine functionalized gold nanoclusters in Spodoptera frugiperda (Sf9) cells
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ORIGINAL PAPER
Uptake and imaging of glycine functionalized gold nanoclusters in Spodoptera frugiperda (Sf9) cells Jinliang Jia1 • Qingle Liu2 • Xiaoyong Jin2 • Hanhong Xu2 Received: 15 April 2020 / Accepted: 11 October 2020 Ó Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Fluorescent gold nanoclusters (Au NCs) have attracted considerable interest in biological application. Here, we reported a novel Au NCs with blue fluorescence, conjugated with glycine (Gly) and rotenone (R). The structure and properties of Au– BSA–Gly–R conjugates were characterized by transmission electron microscopy (TEM), UV–visible, fluorescence spectroscopy, Fourier transform infrared spectroscopy (FTIR) and Zeta potential measurement. The results of fluorescent microscopic imaging revealed that the Au NCs with different ligands were capable of being selectively uptaken by Spodoptera frugiperda (Sf9) cells. Furthermore, the gold content of Au–BSA–Gly–R in Sf9 cells was 2.2 and 2.6 times higher than that of Au–BSA and Au–BSA–R, respectively. It illustrated that nanoparticles after conjugated with Gly could gain specificity to intracellular transportation. This study indicated that the Au NCs after mediated by Gly not only had great potential for applications in insect cell imaging, but also could play an important role of amino acid for agrochemicals delivery in agricultural fields. Keywords Gold nanoclusters Glycine Rotenone Cell imaging Uptake
Introduction Cell imaging and drug delivery by tracking technologies have been widely used for biological phenomena such as transport in cells [1–3], intracellular localization [4] and cell recognition [5]. Fluorescent quantum dots (QDs) also have attracted the attention of researchers because of their unique optical, electronic and physico-chemical
Jinliang Jia and Qingle Liu are co-first authors, who contributed equally to this work.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10876-020-01915-3) contains supplementary material, which is available to authorized users. & Hanhong Xu [email protected] 1
Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China
2
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China
characteristics [6–9]. However, the inherent toxicity prevailing in most of the QDs limits their potential clinical application [10, 11]. In recent years, a promising approach for metal nanoclusters (NCs) as novel fluorescent markers has been widely used because of their biocompatibility, ultrasmall size and nontoxicity [12–15]. Noble metal nanoclusters composed of a few to roughly a hundred atoms, are a new type of luminescent nanomaterials which have consequently attracted a great deal of attention [16, 17]. In contrast to metal nanoparticles (NPs),
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