A Novel Electrochemical Biosensor Based on a Modified Gold Electrode for Hydrogen Peroxide Determination in Different Be
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A Novel Electrochemical Biosensor Based on a Modified Gold Electrode for Hydrogen Peroxide Determination in Different Beverage Samples Navid Nasirizadeh & Saeedeh hajihosseini & Zahra Shekari & Masoud Ghaani
Received: 24 July 2014 / Accepted: 3 November 2014 # Springer Science+Business Media New York 2014
Abstract In the present work, an electrochemical biosensor is constructed based on a Nafion nano composite polymer, toluidine blue (TB) and catalase enzyme-modified gold electrode (AuE). The TB molecules were strongly adsorbed on the Nafion/AuE. The electrochemical properties of this biosensor were examined. Cyclic voltammetry was used at various scan rates to investigate the redox properties of the Nafion and TBmodified AuE (Nafion/TB/AuE). The electron transfer coefficient, α, and the electron transfer rate constant, ks, were found to be 0.48 and 12.1±0.3 s−1 in pH 7.0, respectively. The Nafion, TB, and catalase-modified AuE (Nafion/TB/catalase/AuE) exhibited excellent electrocatalytic response to the reduction of hydrogen peroxide (H2O2). Using cyclic voltammetry, kinetic parameters such as electron transfer coefficient, α, and heterogeneous rate constant, k’, were determined for the reduction of H2O2 at this biosensor surface. Differential pulse voltammetry exhibited two linear dynamic ranges and a detection limit of 0.25 μM for H2O2.
Keywords Biosensor . Nafion . Toluidine blue . Catalase . H2O2
N. Nasirizadeh : S. hajihosseini : Z. Shekari Scientific Society of Nanotechnology, Yazd Branch, Islamic Azad University, Yazd, Iran N. Nasirizadeh (*) Department of Textile Engineering, Yazd Branch, Islamic Azad University, Yazd, Iran e-mail: [email protected] M. Ghaani Department of food science & technology, Science and Research Branch, Islamic Azad University, Yazd, Iran
Introduction Hydrogen peroxide (H2O2) has wide applications in industrial processes as a universal oxidant (Lin et al. 2005). Detection of H2O2 is of interest to many fields such as clinical practice, food industrial process, pharmaceutical, and environmental analysis (Pournaghi-Azar et al. 2010). H2O2 is released into the environment in either large or small quantities as it is widely used in many industrial processes, for example, as the oxidizing, bleaching, or sterilizing agent in packaged materials (Ping et al. 2011). H2O2 is commonly used as a sterilant in packaging materials due to its inherent sporicidal and bactericidal properties (Hsu et al. 2008). However, high concentration of H2O2 would be irritative to the skin and affect human health. Therefore, the determination of H2O2 residues is of practical importance in chemical, biological, and clinical applications, as well as in the food industry. Various analytical techniques have been reported for the determination of H2O2 such as chemiluminescence (Kok et al. 1978; Rocha et al. 2005), photometry (Genfa et al. 1991), fluorimetry (Lazrus et al. 1985), titrimetry (Hurdis and Romeyn 1954), spectrophotometry (Lobnik and Cajlakovic 2001), and electrochemistry (Bai et al. 2007; Hamidi
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