A novel peroxiredoxin from the antagonistic endophytic bacterium Enterobacter sp. V1 contributes to cotton resistance ag
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A novel peroxiredoxin from the antagonistic endophytic bacterium Enterobacter sp. V1 contributes to cotton resistance against Verticillium dahliae Lin Zhang & Ye Tao & Suya Zhao & Xiaoyan Yin & Junmei Chen & Miao Wang & Yingfan Cai & Qiuhong Niu Received: 12 November 2019 / Accepted: 2 August 2020 # Springer Nature Switzerland AG 2020
Abstract [Aims] To reveal the molecular mechanism of endophytic bacteria in Verticillium wilt-resistant cottons and deeply understand the interaction between soil and plants. [Methods] Active tracking screening was used for the isolation of antagonistic strain. Morphological and molecular analyses were conducted to determine its phenotypic and genotypic characteristics. MALDI-TOF/TOF MS was employed to identify the antifungal substance. Functional verification was performed by gene cloning, expression, knockout and complementation. [Results] Enterobacter sp. V1 was isolated from Verticillium-wilt-resistant cotton Xinhai 15, which showed significantly antagonistic abilities against cotton verticillium wilt. A peroxiredoxin named ProV1 with virulent against V. dahliae was obtained. The gene for the peroxiredoxin ProV1 was cloned, and the nucleotide sequence showed 85.5% similarity with peroxiredoxin C (WP 096152328) reported from
E. hormaeche. The purified heterologously expressed ProV1 indicated strong inhibitory activities. Moreover, the suppression of V. dahliae also decreased in proV1 mutant strain compared with wild type strain. The inhibitory activities could be partially restored by a complementation of proV1 gene in the mutant strain. [Conclusion] The results of this study reveal that a novel peroxiredoxin from an endophytic bacterial strain Enterobacter sp. V1 can serve as a virulent factor against fungi V. dahliae, which helps understanding the interaction between endyphytic bacteria and plant pathogenic fungi, and improving the biological control of cotton Verticillium wilt.
Keywords Verticillium wilt of cotton . Vercitillium dahliae Kleb . Endophytic bacteria . Peroxiredoxin . Biological control
Responsible Editor: Hans Lambers Lin Zhang and Ye Tao contributed equally to the work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11104-020-04661-7) contains supplementary material, which is available to authorized users. L. Zhang : M. Wang : Y. Cai (*) State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, Kaifeng, Henan, China e-mail: [email protected]
L. Zhang : Y. Tao : S. Zhao : X. Yin : J. Chen : Q. Niu (*) Department of Life Science and Biotechnology, Nanyang Normal University, Nanyang 473000, People’s Republic of China e-mail: [email protected]
Plant Soil
Introduction Cotton is recognized as the most common crop in more than 30 countries worldwide, including America, China, India, Pakistan and other warm areas (Riaz et al. 2013). The cottonseed is also an important source of edible oil (Ullah et al. 2017). Verticilliu
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