Amycolatopsis jiangsuensis sp. nov., a novel endophytic actinomycete isolated from a coastal plant in Jiangsu, China
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ORIGINAL PAPER
Amycolatopsis jiangsuensis sp. nov., a novel endophytic actinomycete isolated from a coastal plant in Jiangsu, China Ke Xing • Wei Liu • Yue-Ji Zhang • Guang-Kai Bian • Wen-Di Zhang Tomohiko Tamura • Jung-Sook Lee • Sheng Qin • Ji-Hong Jiang
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Received: 7 August 2012 / Accepted: 28 September 2012 / Published online: 5 October 2012 Ó Springer Science+Business Media Dordrecht 2012
Abstract A novel actinomycete, designated strain KLBMP 1262T, was isolated from a coastal plant Dendranthema indicum (Linn.) Des Moul collected from the coastal region of Nantong, Jiangsu Province, in east China and was studied in detail for its taxonomic position. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain KLBMP 1262T is a member of the genus Amycolatopsis. The 16S rRNA gene sequence similarity indicated that strain KLBMP 1262T is most closely related to Amycolatopsis sulphurea DSM 46092T (97.96 %), Amycolatopsis ultiminotia RP-AC36T (97.50 %) and Amycolatopsis jejuensis N7-3T (97.44 %); similarity to other type strains of the genus Amycolatopsis was less than 97.0 %. The Ke Xing and Wei Liu contributed equally to this study.
Electronic supplementary material The online version of this article (doi:10.1007/s10482-012-9823-y) contains supplementary material, which is available to authorized users. K. Xing Y.-J. Zhang G.-K. Bian W.-D. Zhang S. Qin (&) J.-H. Jiang (&) The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou 221116, Jiangsu, People’s Republic of China e-mail: [email protected] J.-H. Jiang e-mail: [email protected]
organism was determined to have chemical and morphological features consistent with its classification in the genus Amycolatopsis such as meso-diaminopimelic acid as the diagnostic diamino acid in the cell wall peptidoglycan and arabinose and galactose as the diagnostic sugars. The predominant menaquinone was determined to be MK-9 (H4). The polar lipids detected were phosphatidylmethylethanolamine, phosphatidylethanolamine, an unknown aminophospholipid, two unknown glycolipids and several unknown lipids. The major fatty acids were found to be C16:0, iso-C16:0 and iso-C15:0. DNA–DNA relatedness data, together with phenotypic differences, clearly distinguished the isolate from its closest relatives. On the basis of these phenotypic and genotypic data, the isolate is considered to represent a novel species, for which the name A. jiangsuensis sp. nov. is proposed. The type strain is KLBMP 1262T (=KCTC 19885 T = NBRC 108679T).
T. Tamura NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan J.-S. Lee Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yuseong-gu, Daejeon 305-806, Republic of Korea
W. Liu Department of Pediatrics, Anyang Children’s Hospital, Anyang 455000, People’s Republic of China
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