Microvirga pakistanensis sp. nov., a novel bacterium isolated from desert soil of Cholistan, Pakistan
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ORIGINAL PAPER
Microvirga pakistanensis sp. nov., a novel bacterium isolated from desert soil of Cholistan, Pakistan Arshia Amin1,2,3 · Iftikhar Ahmed2,4 · Neeli Habib1 · Saira Abbas2,4 · Fariha Hasan3 · Min Xiao5 · Wael N. Hozzein7 · Wen‑Jun Li1,5,6
Received: 7 March 2016 / Revised: 27 May 2016 / Accepted: 31 May 2016 © Springer-Verlag Berlin Heidelberg 2016
Abstract A Gram-negative, non-spore-forming, nonpigmented, strictly aerobic and non-motile short rod bacterium, designated NCCP-1258T, was isolated from Cholistan desert soil, Bahawalpur, Pakistan. Growth of strain NCCP-1258T was observed at pH range 6.5–9.5 (optimum 7.5–8.5) and temperature range 20–45 °C (optimum 40 °C), and it tolerated 0–2 % NaCl (optimum 0.5 %, w/v). Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain NCCP-1258T belongs to genus Microvirga and is most closely related to Microvirga lotononidis (98.0 %), Microvirga vignae (97.4 %), Microvirga lupini (97.2 %), Microvirga zambiensis (97.2 %) and Microvirga flocculans (97.1 %). Analysis of the concatenated sequences of four housekeeping gene loci (dnaK, gyrB, recA and rpoB) also confirmed the Communicated by Erko Stackebrandt.
placement of strain NCCP-1258T within the genus Microvirga. DNA–DNA relatedness values of NCCP-1258T with above-mentioned type strains were less than 42 %. The DNA G+C content of strain NCCP-1258T was 64.3 mol%. Chemotaxonomic data (predominant menaquinone system was Q-10; major fatty acids were C16:0, C18:1 ω7c and C19:0 cyclo ω8c; the polar lipid profile contained diphosphatidylglycerol, phosphatidylcholine, phosphatidyl dimethyl ethanolamine and phosphatidyl ethanolamine) also supported the affiliation of strain NCCP-1258T to the genus Microvirga. On the basis of physiological and biochemical characteristics, phylogenetic analyses and DNA–DNA relatedness, strain NCCP-1258T can be distinguished from the closely related taxa and thus represents a novel species of the genus Microvirga, for which the name Microvirga pakistanensis sp. nov. is proposed with the type strain NCCP1258T (=CGMCC 1.15074T = KCTC 42496T).
Electronic supplementary material The online version of this article (doi:10.1007/s00203-016-1251-3) contains supplementary material, which is available to authorized users. 5
State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, People’s Republic of China
6
Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Üru˝mqi 830011, People’s Republic of China
7
Bioproducts Research Chair (BRC), College of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia
* Iftikhar Ahmed [email protected] * Wen‑Jun Li [email protected] 1
Yunnan Institute of Microbiology, Yunnan University, Kunming, People’s Republic of China
2
Institute of Microbial Culture Collection of Pakistan (IMCCP), National Agricultu
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