Advances in pesticide biosensors: current status, challenges, and future perspectives

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Advances in pesticide biosensors: current status, challenges, and future perspectives Shaoqin Liu & Zhaozhu Zheng & Xinyu Li

Received: 29 February 2012 / Revised: 12 July 2012 / Accepted: 24 July 2012 # Springer-Verlag 2012

Abstract Public concern over pesticide residues has been increasing dramatically owing to the high toxicity and bioaccumulation effects of pesticides and the serious risks that they pose to the environment and human health. It is therefore crucial to monitor pesticide residues by using various analytical methods and techniques, especially highly sensitive, highly selective, simple, rapid, costeffective, and portable ones. Biosensor strategies have become research hotspots and ideal candidates for pesticide detection, having such features as high sensitivity, fast response, robustness, low cost and miniaturization, as well as in situ and real-time monitoring. This review covers advances in the design and fabrication of biosensors for pesticide detection since 2005. Special emphasis is placed on the state-of-art selection of receptors, the use of different transduction techniques and fast screening strategies, and the application of various biosensors developed in food and environmental safety. Both advantages and drawbacks of these techniques are then summarized. Finally, challenges, strategies, and perspectives in further developing pesticide biosensors are also discussed.

S. Liu (*) : Z. Zheng : X. Li Key Laboratory of Microsystems and Microstructures Manufacturing (Ministry of Education), Harbin Institute of Technology, Harbin 150080, China e-mail: [email protected] S. Liu e-mail: [email protected] S. Liu : Z. Zheng : X. Li School of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, China

Keywords Pesticide residue detection . Electrochemical biosensor . Optical biosensor . Enzyme biosensor . Immunosensor Introduction Pesticides, in general, are chemicals used worldwide in agricultural production to destroy or control weeds, insects, fungi, and other pests [1]. Despite their merits, pesticides are considered to be some of the most dangerous environmental contaminants because of their ability to accumulate and their long-term effects on living organisms. The presence of pesticides in the environment is particularly hazardous, and exposure to these pesticides leads to several health problems that range from asthma attacks, skin rashes, and chronic disorders to neurological diseases [2–5]. In response of their high toxicity and the serious risk that they pose to the environment and human health, intense research efforts have been directed over the years to the development of sensitive and selective schemes for detection of pesticides. Currently, over 800 active ingredients, which belong to more than 100 substance classes, are present in a wide range of commercial pesticides. Organochlorine, organophosphorus (OP), and organonitrogen pesticides (referring to carbamates, triazines, and their derivatives) are the most important groups. Among them, organo

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