Auditory and Vestibular Research Methods and Protocols
This second edition expands upon the previous volume with new and updated chapters. Auditory and Vestibular Research: Methods and Protocols, Second Edition guides readers through protocols on cell culture, tissue engineering, nanotechnology, high-throughp
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Bernd Sokolowski Editor
Auditory and Vestibular Research Methods and Protocols Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Auditory and Vestibular Research Methods and Protocols Edited by
Bernd Sokolowski Department of Otolaryngology—Head and Neck Surgery, Morsani College of Medicine, University of South Florida, Tampa, FL, USA
Editor Bernd Sokolowski Department of Otolaryngology—Head and Neck Surgery Morsani College of Medicine University of South Florida Tampa, FL, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3613-7 ISBN 978-1-4939-3615-1 (eBook) DOI 10.1007/978-1-4939-3615-1 Library of Congress Control Number: 2016942277 © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Cover illustration: Cover photo shows a confocal image (Leica SP5) of afferents (Neurovue Orange, red) and efferents (Neurovue Maroon, green) to the basal turn of the organ of Corti in a postnatal mouse. Image provided by B. Fritzsch. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York
Preface Seven years have passed since the last edition of Auditory and Vestibular Research, Methods and Protocols. During this period of time, technological advances and research findings in the field continued at a rapid pace. Thus, no single edition could encompass all the different types of experiments and protocols conducted in the auditory and vestibular fields. With this, the second edition of Auditory and Vestibular Research, Methods and Protocols, we expand the previous volume from three to seven major sections. Here, we introduce new protocols that encompass cell culture, tissue engineering, nanotechnology, high-throughput
