Effect of Naturally Occurring Biogenic Materials on Human Dental Pulp Stem Cells (hDPSC): an In Vitro Study.
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ORIGINAL RESEARCH
Effect of Naturally Occurring Biogenic Materials on Human Dental Pulp Stem Cells (hDPSC): an In Vitro Study. Prasanna T. Dahake 1 Vijay M. Kumbar 2
&
Vinod V. Panchal 1 & Yogesh J. Kale 1 & Mahesh V. Dadpe 1 & Shrikant B. Kendre 1 &
Received: 12 May 2020 / Revised: 24 July 2020 / Accepted: 17 August 2020 # The Regenerative Engineering Society 2020
Abstract Objectives This study aimed to evaluate and compare the cell viability and cell differentiation potential together with the antiinflammatory potential of coral (Now Foods Calcium Carbonate Powder, HerbsPro, India) and Biodentine™ (Septodont, StMaur-des-Fosses, Cedex, France) on human dental pulp stem cells (hDPSC). Materials and Methods Human dental pulp stem cells (hDPSC) were segregated and cultured from the dental pulp of healthy teeth undergoing therapeutic extraction. For assessing cell proliferation potential of coral and Biodentine, the agents were taken at a concentration (800, 400, 200, 100, 50 μg/mL) and for cell differentiation potential a concentration of 400 μg/mL respectively. MTT assay was utilized to evaluate proliferation, while differentiation was assessed using Alizarin Red staining. The antiinflammatory potential of Biodentine and coral was likewise evaluated using gelatin zymography by detecting MMP-2 and MMP-9 matrixin. Results The highest cell proliferation for coral and Biodentine was observed at 200 μg/mL (108.82 ± 2.09% and 107.32 ± 1.04%, respectively), which was statistically significant (p = 0.043), while cell differentiation potential at 400 μg/mL was 127.70 ± 4.26% for coral and 142.96 ± 4.06% for Biodentine. However, anti-inflammatory activity against MMP-2 seen with coral was 68 ± 1.14% and 85.0 ± 3.05 for Biodentine, while against MMP-9 was 30 ± 1.69 for coral and 65 ± 2.74 for Biodentine. Conclusions Coral has comparable potential to enhance proliferation and differentiation of hDPSC into the functional and utilitarian cells like odontoblasts or osteoblast and to harbor anti-inflammatory activity similar to Biodentine. Keywords Anti-inflammatory . Biodentine . Cell differentiation . Cell proliferation . Coral . hDPSC
Introduction * Prasanna T. Dahake [email protected] Yogesh J. Kale [email protected] Mahesh V. Dadpe [email protected] Shrikant B. Kendre [email protected] Vijay M. Kumbar [email protected] 1
Department of Pediatric and Preventive Dentistry, MIDSR Dental College and Hospital, Latur, Maharashtra 413512, India
2
Central Research Laboratory, Maratha Mandal’s NGH Institute of Dental Sciences and Research Centre, Belgaum, Karnataka 590019, India
The dental pulp is occasionally exposed by dental caries, trauma, or iatrogenic injury in clinical practice. Untreated pulp or chronic dentinal exposure can cause pulp necrosis in bacterial infections and can undermine the integrity of the tooth structure. Additionally, dental tissues under stress are associated with a high risk of tooth fracture. Indirect pulp capping, direct pulp capping, and partial or full coronal pulpotomy
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