Bacteria Mediated Synthesis of Iron Oxide Nanoparticles and Their Antibacterial, Antioxidant, Cytocompatibility Properti
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ORIGINAL PAPER
Bacteria Mediated Synthesis of Iron Oxide Nanoparticles and Their Antibacterial, Antioxidant, Cytocompatibility Properties Shahnaz Majeed1 • Mohammed Danish2 • Mohamad Nasir Mohamad Ibrahim3 • Siti Hajar Sekeri3 Mohammed Tahir Ansari4 • Anima Nanda5 • Gulzar Ahmad5
•
Received: 25 February 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Iron oxide nanoparticles (IONPs) were synthesized from the Proteus vulgaris ATCC-29905 using the extracellular methodology. The UV–Vis spectrophotometry showed the k-max (maximum absorbance) absorption peak at 310 nm. The FTIR analysis showed amides and other functional groups are associated with IONPs. The TGA results showed less protein loss (DY = 9.045%) at a protein degradation temperature of 71.73 C. The FESEM images showed particles are spherical in shape. The EDX confirms the presence of iron. Zeta potential interface was found to be 79.5 mV that confirms its stability. The TEM results showed particles are spherical with a diameter between 19.23 nm ad 30.51 nm. These IONPs showed good antibacterial activity and also showed good activity against methicillin resistant staphylococcus aureus (MRSA). It showed good antioxidant activity. These IONPs exhibits good cytotoxic effect against U87 MG—glioblastoma cancer cells, showed IC50 value at 250 lg/ml compared with healthy L-132 cells. Scratch assay showed IONPs inhibit the cell migration of the HT-29 cancer cells. The nanoparticles synthesized from bacteria are safe and non-hazardous. It was expected that these IONPs could become a potential anticancer and antibacterial agent, and in the future it opens a new path for treating the cancer patients. Keywords Proteus vulgaris Anticancer activity antibacterial activity Antioxidant activity Scratch assay
Introduction
& Shahnaz Majeed [email protected] 1
Faculty of Pharmacy and Health Sciences, Universiti Kuala Lumpur Royal College of Medicine Perak, 30450 Ipoh, Perak, Malaysia
2
Bioengineering Technology Section, Universiti Kuala Lumpur Malaysian, Institute of Chemical and Bioengineering Technology (MICET), Lot 1988, Kawasan Perindustrian Bandar Vendor, Taboh Naning, 78000 Alor Gajah, Melaka, Malaysia
3
School of Chemical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia
4
School of Pharmacy, University of Nottingham Malaysia, 43500 Semenyih, Malaysia
5
Department of Biomedical Engineering, Sathyabama University, Chennai, India
Nanobiotechnology deals with the technology that controls the size of functional materials in the range of 1–100 nm. The potential applications of these nanomaterials, with highly ordered design, showed a promising response by exposing them with proper stimuli [1]. Nanotechnology forms a bridge between biological science and physical science. The designing and formation of nanomaterials is an outcome of the knowledge of material science and its functional properties, which are explored through the understanding of biological science
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