Biological Microarrays Methods and Protocols
Recent developments in microarray technology have changed the landscape of biology and biomedical research, and they have revolutionized RNA and DNA research. In Biological Microarrays: Methods and Protocols, expert researchers explore exciting new develo
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
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Biological Microarrays Methods and Protocols
Edited by
Ali Khademhosseini Department of Medicine, MIT Division of Health Sciences and Tech, Harvard Medical School, Cambridge, MA, USA
Kahp-Yang Suh Department of Mechanical and Aerospace E, Seoul National University, Kwanak-gu, Shinlim-dong 56-1-1, 151-742, Seoul, Korea, Republic of (South Korea) Gwanak-ro 599, Gwanak-gu, Seoul 151-742, Republic of Korea
Mohammed Zourob Biophage Pharma, Montreal, QC, Canada
Editors Prof. Dr. Ali Khademhosseini Department of Medicine MIT Division of Health Sciences and Tech Harvard Medical School Cambridge, MA USA [email protected]
Dr. Mohammed Zourob Biophage Pharma Montreal, QC Canada [email protected]
Kahp-Yang Suh Department of Mechanical and Aerospace E Seoul National University Kwanak-gu, Shinlim-dong 56-1-1, 151-742 Seoul, Korea, Republic of (South Korea) Gwanak-ro 599, Gwanak-gu, Seoul 151-742, Republic of Korea [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-934115-95-4 e-ISBN 978-1-59745-551-0 DOI 10.1007/978-1-59745-551-0 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2010938723 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface Microarrays are spatially ordered arrays with ligands chemically immobilized in discrete spots on a solid matrix, usually a microscope slide. Microarrays are a high-throughput large-scale screening system enabling simultaneous identification of a large number of target molecules (up to several hundred thousand) that bind specifically to the immobilized ligands of the array. Microarrays represent a promising tool fo
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