Microcalorimetry of Biological Molecules Methods and Protocols
This volume provides methods on microcalorimetry approaches to investigate complex biological molecular systems. Chapters guide readers through Differential Scanning Calorimetry (DSC), Isothermal Titration Calorimetry (ITC), and advanced data processing.
- PDF / 6,732,145 Bytes
- 264 Pages / 504.63 x 737.01 pts Page_size
- 88 Downloads / 239 Views
Eric Ennifar Editor
Microcalorimetry of Biological Molecules Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Microcalorimetry of Biological Molecules Methods and Protocols
Edited by
Eric Ennifar Institut de Biologie Moléculaire et Cellulaire, Université de Strasbourg, CNRS, Strasbourg, France
Editor Eric Ennifar Institut de Biologie Mole´culaire et Cellulaire Universite´ de Strasbourg, CNRS Strasbourg, France
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9178-5 ISBN 978-1-4939-9179-2 (eBook) https://doi.org/10.1007/978-1-4939-9179-2 Library of Congress Control Number: 2019935496 © Springer Science+Business Media, LLC, part of Springer Nature 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Although structural biology is key to elucidate molecular architecture of biological molecules, thermodynamic and kinetic data are yet an essential and necessary complement to structural studies for a comprehensive understanding of binding mechanisms and mechanisms of action. Structural data alone, even when associated with sophisticated computational methods, cannot fully define the driving forces for binding interactions or even accurately predict their binding affinities or kinetics. Thermodynamics and kinetics provide quantitative data required to understand these driving forces, the time in which drug and its target associate and dissociate, and for evaluating and understanding at a deeper level the effects
Data Loading...
