Dexamethasone and lidocaine suppress eosinophilopoiesis from umbilical cord blood cells
- PDF / 1,676,224 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 94 Downloads / 220 Views
(2020) 18:24 Muraki et al. Clin Mol Allergy https://doi.org/10.1186/s12948-020-00138-1
Open Access
RESEARCH
Dexamethasone and lidocaine suppress eosinophilopoiesis from umbilical cord blood cells Masato Muraki1,2* , Hirohito Kita1,3 and Gerald J. Gleich1,4
Abstract Background: Eosinophils play an important role in allergic inflammation. Glucocorticosteroids have been used as an anti-inflammatory medication for inflammatory diseases involving eosinophil infiltration. Some effect of nebulized lidocaine has been reported when treating certain patients with asthma, which is also an inflammatory disease. The goal of this study was to examine the effects of dexamethasone and lidocaine on eosinophil proliferation and differentiation using a model of human umbilical cord blood mononuclear cells (UCMC) cultured with IL-5. Methods: UCMC were cultured with IL-5 (5 ng/mL) for 4 weeks. The effects of dexamethasone and lidocaine on the number and morphology of eosinophilic cells were visualized with Wright-Giemsa and cyanide-resistant peroxidase stains. Moreover, the effect on eosinophil-derived neurotoxin (EDN) and eosinophil peroxidase (EPX) contents in cultured cells were evaluated using radioimmunoassay. Results: The number of eosinophilic cells and EDN and EPX content in cultured cells increased in a time-dependent manner in the presence of IL-5. Dexamethasone treatment slightly decreased the number of eosinophilic cells in one week, but this effect was lost in 2–4 weeks. Macrophages in cultured UCMC treated with dexamethasone contained more eosinophil granule proteins. Both EDN and EPX content in cultured cells were reduced by dexamethasone. Lidocaine decreased the number of eosinophilic cells and reduced both EDN and EPX contents in cultured cells. Conclusions: Dexamethasone suppressed the production of eosinophil granule proteins and may also induce apoptosis of eosinophils, while lidocaine suppresses eosinophilopoiesis. Keywords: Dexamethasone, Lidocaine, Eosinophilopoiesis, Eosinophil-derived neurotoxin, Eosinophil peroxidase, Umbilical cord blood Background Eosinophils play an important role in allergic diseases, including asthma. Corticosteroid therapy is effective for treating eosinophilic diseases [1–4]. However, the mechanisms of eosinophil development and survival are only partially understood [5]. Both the inhibitory [6] and
*Correspondence: muraki‑[email protected] 2 Department of Respiratory Medicine and Allergology, Kindai University Nara Hospital, 1248‑1 Otoda‑cho, Ikoma, Nara 630‑0293, Japan Full list of author information is available at the end of the article
stimulatory [7] effects of glucocorticoids are reported; however, their effect on eosinophilopoiesis is still unclear. Inhaled or systemic corticosteroids are ineffective treatments in many patients with severe asthma, and few treatment options exist for patients with steroid-resistant asthma [8], although inhaled corticosteroids are the initial controllers administered for treating asthma [9]. IL-5 is the main cytokine targeting eosino
Data Loading...
