Mouse Cell Culture Methods and Protocols
This volume describes cell culture protocols for the maintenance, propagation, manipulation, and analysis of primary explanted cells from various mouse organ systems. After introductory chapters that examine the applicability of mouse models as a discover
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Ivan Bertoncello Editor
Mouse Cell Culture Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Mouse Cell Culture Methods and Protocols
Edited by
Ivan Bertoncello Lung Health Research Centre, Department of Pharmacology and Therapeutics, University of Melbourne, Melbourne, Victoria, Australia
Editor Ivan Bertoncello Lung Health Research Centre Department of Pharmacology and Therapeutics University of Melbourne Melbourne, Victoria, Australia
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9085-6 ISBN 978-1-4939-9086-3 (eBook) https://doi.org/10.1007/978-1-4939-9086-3 Library of Congress Control Number: 2019930653 © Springer Science+Business Media, LLC, part of Springer Nature 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Mouse models have long underpinned discovery in the biomedical sciences and the development of enabling technologies for the systematic analysis of cellular and molecular mechanisms of organ development, regeneration, and repair. In particular, mouse cell culture systems continue to play an important role in identifying key factors, genes, and pathways regulating cell lineage commitment and differentiation and validating potential cellular and molecular targets which could be exploited to develop novel therapies for intractable diseases. This compendium describes recently devised and refined best-practice cell culture protocols for the maintenance, propagation, manipulation, and analysi
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