Mouse Cell Culture Methods and Protocols
Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Protocols, expert researcher
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MO L E C U L A R BI O L O G Y
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For other titles published in this series, go to www.springer.com/series/7651
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Mouse Cell Culture Methods and Protocols
Edited by
Andrew Ward University of Bath, Bath, UK
David Tosh University of Bath, Bath, UK
Editors Andrew Ward Department of Biology & Biochemistry University of Bath Claverton Down Bath United Kingdom BA2 7AY [email protected]
David Tosh Department of Biology & Biochemistry University of Bath Claverton Down Bath United Kingdom BA2 7AY [email protected] [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-58829-772-3 e-ISBN 978-1-59745-019-5 DOI 10.1007/978-1-59745-019-5 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2010921109 © Springer Science+Business Media, LLC 2010 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface Techniques for the isolation, maintenance and growth of tissues normally found in a multicellular organism can be traced back for over 100 years. The pioneering developmental biologist Wilhelm Roux is generally attributed with the first reported tissue culture experiment after maintaining an explant of chick medullary plate for several days in a warm saline solution. This experiment recognised the need to provide cultured cells with conditions that resemble their normal environment, including an optimal temperature and an isotonic medium, and could be considered the founding principle of tissue culture. Tissue culture has become more sophisticated since then mainly through the ability to increasingly recognise the conditions needed to support specific cell types and, indeed, to instruct their behaviour in vitro. Tissue culture experiments have been used to address many different biological questions. Most obviously, cultured cells have been used to study the properties of the tissues from which they are derived. The accessibility of cultured cells, combined with the ability to expand a homogeneous cell population from a relatively limited source, opens up a wealth of possibilities for researchers. Cultured cells have been used to manufacture protein products, and, as a test bed for ne
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