Epithelial Cell Culture Methods and Protocols

This detailed book explores the most current techniques to study systems and epithelial cell culture. Beginning with an overview, the volume then continues to detail systems that seek to mimic the three-dimensional organization, epithelial cells from diff

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Mario Baratta Editor

Epithelial Cell Culture Methods and Protocols

Methods

in

M o l e c u l a r B i o lo g y

Series Editor: John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Epithelial Cell Culture Methods and Protocols

Edited by

Mario Baratta Department of Veterinary Science, University of Turin, Turin, Italy

Editor Mario Baratta Department of Veterinary Science University of Turin Turin, Italy

ISSN 1064-3745     ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-8599-9    ISBN 978-1-4939-8600-2 (eBook) https://doi.org/10.1007/978-1-4939-8600-2 Library of Congress Control Number: 2018946598 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.

About the Editor Mario Baratta is full professor of veterinary physiology at the University of Turin (IT). He received his master’s degree in veterinary medicine at the University of Parma (IT) and his PhD in neuroendocrinology of animal farm at the University of Bologna (IT). Today he is the coordinator of the Graduate School program in Veterinary Science for Animal Health and Food Safety, University of Turin. His main interests are the biology of adult stem cells in different tissues, and he has developed specific knowledge in functional assessment systems of epithelial cells and cell differentiation in mammary gland and in muscle in animal science.

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Preface Epithelial tissues line the cavities and surfaces of blood vessels and organs throughout the body, and epi