Plant Chromosome Engineering Methods and Protocols
With an increasing human population and a decreasing amount of arable land, creative improvements in agriculture will be a necessity in the coming decades to maintain or improve the standard of living. In Plant Chromosome Engineering: Methods and Protocol
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
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Plant Chromosome Engineering Edited by
James A. Birchler Division of Biological Sciences University of Missouri Columbia, MO, USA
Editor James A. Birchler, Ph.D. Division of Biological Sciences University of Missouri Columbia, MO, USA [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61737-956-7 e-ISBN 978-1-61737-957-4 DOI 10.1007/978-1-61737-957-4 Springer New York Dordrecht Heidelberg London © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface With a stable or decreasing amount of arable land on earth but with a continuing increase in human population, creative improvements in agriculture will be needed in the coming decades to maintain or improve the standard of living. Novel approaches to the production of food, feed, fuel, fiber, and pharmaceuticals will be needed, and the modification of crops and other plant species is one means to achieve such a goal. This volume on plant chromosome engineering includes reviews and protocols for transformation procedures, chromosome painting, production of engineered minichromosomes, gene targeting and mutagenesis, site-specific integration, gene silencing, protein expression, chromosome sorting and analysis, protocols for generating chromosomal rearrangements, enhancer trapping, and means of studying chromosomes in vivo. Collectively, these chapters touch upon the spectrum of tools currently available for modifying plant genomes and chromosomes and provide the foundation for future developments.
James A. Birchler
Columbia MO
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Contents Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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