Combating atherosclerosis with targeted Diosmin nanoparticles-treated experimental diabetes
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PRECLINICAL STUDIES
Combating atherosclerosis with targeted Diosmin nanoparticles-treated experimental diabetes Hendawy OM 1 & Mehrez E. El-Naggar 2 & Mona El-Banna 3 & Moustafa M. G. Fouda 2 & Sarah I. Othman 4 & Ahmed A. Allam 5 & Osama M. Morsy 6 Received: 16 January 2020 / Accepted: 27 January 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Summary Diabetes with poor glycemic control is accompanying with an increased risk of disease namely atherosclerotic cardiovascular. Diosmin (DSN), which is obtained from citrus fruit used to assist the treatment of hemorrhoids or chronic venous atherosclerosis diseases, has an antioxidant, anti-hyperglycemic and anti-inflammatory effect. DSN is characterized by poor water solubility which limits its absorption by the gastrointestinal tract. To overcome this limitation, this study was designed to increase DSN bioavailability and solubility, through its loading on polymeric matrix; hydroxypropyl starch (HPS) and Poly lactide–glycolidechitin (PLGA/chitin) to prepare Diosmin nanoparticles (DSN-NPs). Two methods were used to prepare DSN- NPs; Emulsion– solvent evaporation and Acid–base neutralization followed by further assessment on diabetes induced atherosclerosis The study was conducted on 50 animals assigned into 5 groups with 10 animals in each group: Group I: Normal rats received only normal saline, Group II: Diabetic rats, Group III: diabetic rats received oral DSN, Group IV: diabetic rats received DSN loaded HPS, Group V: diabetic rats received DSN loaded PLGA/chitin. Levels of total cholesterol, triglycerides, HDL-cholesterol, insulin, MDA and NO. plasminogen activator inhibitor-1 PAI-1), Paraoxonase-1(PON1), transforming growth factor-β1 (TGF-β1), NFB and Ang II were estimated. Our study revealed that, there was statistically significant difference between DSN treated group compared with DSN loaded HPS treated group and DSN loaded PLGA/chitin. Furthermore, the results obtained clearly disclosed no statistically significant difference between DSN loaded PLGA/chitin and control group exhibited DSN loaded PLGA/chitin has the higher ability to counteract the atherosclerosis factors induced by diabetes in all rats. Keywords Diosmin . Emulsion–solvent evaporation . Acid–base neutralization . Nanoparticles . Atherosclerotic cardiovascular
Introduction Atherosclerosis is considered as chronic lipid derived inflammatory arterial disease embodied in high risk plaque formation in the large arteries [1]. Diabetes mellitus (DM) is a vastly prevalence metabolic disorder associated by higher blood
* Mehrez E. El-Naggar [email protected] * Moustafa M. G. Fouda [email protected] 1
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Faculty of Pharmacy, Jouf University, Saudi Arabia-Lecturer of Clinical Pharmacology, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt Pre-Treatment and Finishing of Cellulosic Fabric Department, Textile Research Division, National Research Center, 33 El- Behooth St, Dokki, Giza 12311, Egypt
sugar level over prolonged duration causing intimal blood vessel da
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