Methods in Mouse Atherosclerosis
This volume reviews available mouse models of atherosclerosis, basic aspects of disease initiation and progression, and the visualization of atherosclerotic lesions with invasive and non-invasive techniques that are widely used both in basic research and
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Vicente Andrés Beatriz Dorado Editors
Methods in Mouse Atherosclerosis
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Methods in Mouse Atherosclerosis Edited by
Vicente Andrés Laboratory of Molecular and Genetic Cardiovascular Pathophysiology, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain
Beatriz Dorado Laboratory of Molecular and Genetic Cardiovascular Pathophysiology, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain
Editors Vicente Andrés Laboratory of Molecular and Genetic Cardiovascular Pathophysiology Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) Madrid, Spain
Beatriz Dorado Laboratory of Molecular and Genetic Cardiovascular Pathophysiology Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) Madrid, Spain
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2928-3 ISBN 978-1-4939-2929-0 (eBook) DOI 10.1007/978-1-4939-2929-0 Library of Congress Control Number: 2015948251 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Atherosclerosis and associated cardiovascular disease (CVD), most prominently manifesting as myocardial infarction or stroke, constitute the main cause of morbidity and mortality in industrialized countries. In spite of major advances in our understanding of the genetic, molecular, and cellular determinants of CVD, its incidence and prevalence are expected to increase significantly over the coming decades owing to the progressive aging of our societies (agin
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