Complete mitochondrial genome of albino cyprinid loach, Misgurnus anguillicaudatus (Cypriniformes: Cobitidae)
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TECHNICAL NOTE
Complete mitochondrial genome of albino cyprinid loach, Misgurnus anguillicaudatus (Cypriniformes: Cobitidae) Sang Yoon Lee1 · In‑Chul Bang2 · Yoon Kwon Nam1
Received: 17 August 2017 / Accepted: 28 August 2017 © Springer Science+Business Media B.V. 2017
Abstract Albinism, a congenital genetic mutation, is rarely found in natural populations of cyprinid loach Misgurnus anguillicaudatus (Cypriniformes; Cobitidae). The entire mitogenome of albino M. anguillicaudatus (16,647 bp), which is found in a tributary of Korean river, represents a structural organization typical of a vertebrate mtDNA comprising of 13 protein coding genes (PCGs), two ribosomal RNAs (rRNAs), 22 transfer RNA (tRNA) genes, one replication origin ( OL), and one control region (D-loop). Phylogenetic analysis suggests that albino cyprinid loach in this study corresponds to the “introgressed” mitochondrial type of M. anguillicaudatus based on the mtDNA introgression hypothesis in genus Misgurnus. Mitogenome information from this study could be a useful basis for conservation and phylogenetics of the natural albino cyprinid loaches. Keywords Albino cyprinid loach · Misgurnus anguillicaudatus · Mitogenome · Conservation Cyprinid loach, Misgurnus anguillicaudatus (Cypriniformes; Cobitidae), a small-sized freshwater fish species, is a highly valued, aquaculture-relevant food fish in East Asian countries. This loach species has also been given much attention as an important model organism to study developmental biology (Fujimoto et al. 2006), polyploidy evolution (Zhou et al. 2014) and genetic breeding (Arai * Yoon Kwon Nam [email protected] 1
Department of Marine Bio‑Materials & Aquaculture, Pukyong National University, Busan 48513, South Korea
2
Department of Marine Biotechnology, Soonchunhyang University, Asan 31538, South Korea
2003). Meanwhile, the model fish carrying albinism, a rare genetic mutation, is an invaluable experimental platform for phenotype marker-assisted research (Morishima et al. 2011), visible reporter-based heterologus expression assay (Thomas et al. 2012) and also theoretical study on pigmentation mechanism (Zou et al. 2015). Further, albino fish have also received a long-lasting interest as a potential resource to generate a novel fish breed in ornamental market. Recently, natural albino cyprinid loaches were identified in Southern part of Korea, and herein, its complete mitogenome was sequenced in order to obtain essential genetic information. The mitogenome data from this study could be a fundamental basis to address genetic identity and diversity in future conservation program of this rarely occurring, natural albino loach. The albino M. anguillicaudatus samples were collected from the Mangyeong River, Jeollabuk-do, South Korea. Diploidy status of the albino loaches was verified with flow cytometric analysis of cellular DNA content in erythrocytes (Nam et al. 2004). Total DNA was isolated from caudal fin by using conventional SDS/proteinase K method followed by organic extraction and ethanol pre
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