The complete mitochondrial genome of Chinese Sarcocheilichthys kiangsiensis (Cypriniformes: Cyprinidae: Sarcocheilichthy
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TECHNICAL NOTE
The complete mitochondrial genome of Chinese Sarcocheilichthys kiangsiensis (Cypriniformes: Cyprinidae: Sarcocheilichthys) Qin Guo1 · Xiong‑Jun Liu1 · Xiao‑Ping Wu1,2 · Shan Ouyang1
Received: 23 December 2016 / Accepted: 3 February 2017 © Springer Science+Business Media Dordrecht 2017
Abstract Sarcocheilichthys kiangsiensis: is a threatened (IUCN: Least Concern) and native fish in China. This study deals with the complete mitochondrial genome of Sarcocheilichthys kiangsiensis by using next-generation sequencing. The full length of the circular genome is 16,672 bp. It consists of 37 typical animal mitochondrial genes including 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes and 2 ribosomal RNA (rRNA) genes and a control region. The overall nucleotide composition is: 29.7% A, 25.7% T, 27.4% C, and 17.2% G, with a total A + T content of 55.4%. Keywords Sarcocheilichthys kiangsiensis · Mitochondrial genome · Next-generation sequencing Sarcocheilichthys belongs to subfamily Gobioninae within the Cyprinidae comprising 10 species/sub-species widely distributed in East Asia, and is a kind of small freshwater fish, inhabiting middle or low layer of rivers, streams and lakes. Sarcocheilichthys represents a valuable model for understanding the speciation of freshwater fishes in East Asia (Zhang et al. 2008), which have an unusual mating system that involves laying their eggs inside the mantle cavity of freshwater mussels (Luo et al. 1977; Zhang * Shan Ouyang [email protected] Xiao‑Ping Wu [email protected] 1
School of Life Sciences, Nanchang University, Nanchang, People’s Republic of China
2
Center for Watershed Ecology, Institute of Life Science, Nanchang University, Nanchang, People’s Republic of China
et al. 2008). Sarcocheilichthys kiangsiensis Nichols, 1930, is mainly distributed in the south of the Yangtze River in China. Unfortunately, habitat destruction and human-associated insults (dam construction, sand excavation, overfishing of fish) have led to rapid population resource declines of Sarcocheilichthys kiangsiensis, which made it threatened (IUCN: Least Concern). However, there are few researches for Sarcocheilichthys kiangsiensis, on reproductive biology (Li et al. 2012) and molecular phylogenetic relationship of the genus Sarcocheilichthys (Zhang et al. 2008). In this study, we report the complete mitochondrial genome of Sarcocheilichthys kiangsiensis based on next-generation sequencing. The newly sequenced complete mitochondrial genome sequence will provide useful information on conservation genetic and the future ecological assessments of population genetics for the fish. The specimens were transported alive from Ganjiang River (Jiangxi province, China). The total DNA was extracted from muscular tissue by using Wizard SV Genomic DNA Purification System (Promega, Madison, WI) and kept at −20 °C. Mitochondrial genome data were generated by using long-range PCR and primer-walking method. The complete mitochondrial genome of Sarcocheilichthys kiangsiensis was 16,672 bp in len
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