Complete mitochondrial genome of Qingyang donkey ( Equus asinus )
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TECHNICAL NOTE
Complete mitochondrial genome of Qingyang donkey (Equus asinus) Xian Guo1 · Pengjia Bao1 · Jie Pei1 · Xuezhi Ding1 · Chunnian Liang1 · Ping Yan1 · Dengxue Lu2
Received: 21 November 2016 / Accepted: 27 December 2016 © Springer Science+Business Media Dordrecht 2017
Abstract The present study aimed to report the entire mitochondrial genome of Qingyang donkey (Equus asinus). Donkeys play an important role in the daily lives of human beings. The population of Chinese donkey breeds has undergone an enormous decline with the advance of agricultural mechanization, rapid development of transportation and introduction of new different breeds over the past few decades. As a result, a large number of donkey breeds are close to extinction, especially Qingyang donkey. Mitochondrial DNA is used as a molecular tool in research on biodiversity conservation and sustainable utilization of biological resources. The circular genome sequenced using the Illumina Hiseq 2500 platform was 16,568 base pairs (bp), containing 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a non-coding control region. The initiation site of the L-strand replication origin, 32 bp in length, was between tRNA-Asn and tRNA-Cys. The base composition of the genome was A (32.4%), C (28.7%), G (13.2%) and T (25.7%) with an A+T content of 58.1%. Molecular phylogeny demonstrated two separated clades in Equidae and the presence of a distant relationship between the domestic donkey breeds and Asian wild asses, indicating that domestic donkeys might not probably have originated from Asian wild asses. However, African wild asses were grouped as a * Ping Yan [email protected] * Dengxue Lu [email protected] 1
Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, 335 Jiangouyan, Qilihe District, Lanzhou 730050, Gansu, China
2
Institute of Biology, Gansu Academy of Sciences, 197 Dingxi South Road, Chengguan District, Lanzhou 730000, China
sister to domestic donkeys. This study presented the complete mitochondrial genome of E. asinus, providing the important genetic background of protection and adequate utilization of domestic donkey breeds. Keywords Equus asinus · Complete mitogenome · Illumina sequencing
Qingyang donkey (Equus asinus) in the family Equidae belongs to a medium-sized domestic donkey breed and is commonly used to transport goods and produce meat. However, unfortunately, its number recently has fallen sharply for the sake of economic benefits and owing to neglect of germplasm conservation. Mitochondrial DNA (mtDNA) has been emerged as a well-accepted molecular tool with extensive applications including origin and evolution, genetic relationships and population genetic structure of livestock (Lippold et al. 2011; Achilli et al. 2012). The complete mitogenome of Qingyang donkey (GenBank: KX683425) was sequenced, assembled and characterized in this study to provide molecular genetic evidence for the conservation of this breed. The high-quality total genomic DNA was isolated from the blood
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