Draft Genome Sequence of a Potential Organic Phosphorus-Degrading Bacterium Brevibacterium frigoritolerans GD44, Isolate
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Draft Genome Sequence of a Potential Organic Phosphorus‑Degrading Bacterium Brevibacterium frigoritolerans GD44, Isolated from Radioactive Soil in Xinjiang, China Mengmeng Jin1 · Qianru Zhao1 · Zhi Zhou1 · Liying Zhu2 · Zhidong Zhang3 · Ling Jiang4 Received: 11 November 2019 / Accepted: 16 May 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Brevibacterium frigoritolerans, a strain quite potential use in environmental pollution, is also able to degrade the pesticide phorate. Here, we report a strain isolated from radioactive soil in the Xinjiang Uygur Autonomous Region of China. The genome of strain GD44 encompasses 5,471,331 base pairs with a GC content of 40.42%. The sequence was assembled into 1985 open reading frames (ORFs) encoding 5053 proteins. Sequence analysis identified the genes encoding enzymes related to the degradation of organophosphorus compounds such as esterase, phosphotransferase, C–P lyase, and alkaline phosphatase. The nitrate reductase gene was also found in GD44, which was associated with biosynthesis of silver nanoparticles used for bacteriostat. In addition, Antibiotic Resistance Ontology (ARO) genes accounted for 10.6%, including the vancomycin resistance gene cluster. Therefore, the whole-genome sequence of B. frigoritolerans GD44 will be beneficial for identifying and analyzing genes utilized for soil remediation and antibacterial agent, which will provide genetic evaluation for potential application in the future.
Introduction Crop pests and diseases are among the agricultural hazards that can affect the development of the national economy most seriously, especially by causing heavy losses of agricultural production. Phorate is an efficient organophosphorus Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02037-9) contains supplementary material, which is available to authorized users. * Zhi Zhou [email protected] * Ling Jiang [email protected] 1
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 210009, People’s Republic of China
2
College of Chemical and Molecular Engineering, Nanjing Tech University, Nanjing 210009, People’s Republic of China
3
Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Xinjiang Uigur Autonomous Region, Urumqi, People’s Republic of China
4
College of Food Science and Light Industry, Nanjing Tech University, Nanjing 210009, People’s Republic of China
insecticide, but its residues can cause soil contamination and may seriously damage the ecosystem [1–4]. Hydrolase and other enzymes play important roles in the degradation pathways of organophosphorus insecticides, usually in combination [5, 6]. Brevibacterium frigoritolerans has the ability to degrade phorate, reaching a degradation rate of more than 90% [1, 7, 8]. Except for the degradation of organic phosphorus, the bacteria of this genus also have other important applications. For example, B. frigoritolerans strain HSB-15 is an en
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