Fabrication of homogeneous waffle-like silver composite substrate for Raman determination of trace chloramphenicol
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ORIGINAL PAPER
Fabrication of homogeneous waffle-like silver composite substrate for Raman determination of trace chloramphenicol Dongfang Xiao 1 & Zhishun Jie 1 & Zhiyuan Ma 1 & Ye Ying 1
&
Xiaoyu Guo 1 & Ying Wen 1 & Haifeng Yang 1
Received: 12 June 2020 / Accepted: 25 September 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract Waffle-like anodized aluminum oxide homogeneously immobilized with Ag nanoparticles (AAO/Ag) is rationally designed and fabricated as surface-enhanced Raman scattering (SERS) substrate. The as-prepared SERS substrate is characterized with transmission electron microscope (TEM), scanning electron microscopy (SEM), UV–Vis spectrophotometer, and Fourier transform infrared spectrometer (FT-IR). The AAO/Ag substrate shows good uniformity of the Raman signals (RSD = 7.02%) due to waffle-like AAO supporting the well-dispersed Ag nanoparticles. For real application, the AAO/Ag substrate is used for rapid determination of chloramphenicol (CAP) in honey with low detection limit (4.0 × 10−9 mol L−1) and good linearity from 1.0 × 10−5 to 1.0 × 10−8 mol L−1 based on the SERS peak at 1348 cm−1. The better accumulation in the short pore path of AAO improves the target molecule approaching into the vicinity of hot spots of Ag nanoparticles. The high selectivity for CAP is attributed to the strong interaction between -NO2 group in CAP and the composite substrate. Keywords Anodized aluminum oxide . Ag nanoparticles . In situ reduction . Trace chloramphenicol determination . Food safety
Introduction Chloramphenicol (CAP) is a bacteriostatic broad-spectrum antibiotic, prolonged exposure of which leads to drug resistance [1]. Its residue causes severe aplastic anemia and gray infant syndrome [2]. CAP in body is hardly degraded in a short time and then is excreted, which further contaminates the environment [3]. Therefore, in many countries, CAP has been banned as additive in foods and animal treatments [4]. However, due to its low price, the illegal use of CAP always happens [5]. Traditional methods for detection of CAP, including gas chromatography combined with mass spectrometry (GC-MS) [6], molecular imprinting [7], and enzymeElectronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04567-2) contains supplementary material, which is available to authorized users. * Ye Ying [email protected] * Haifeng Yang [email protected] 1
Department of Chemistry, Key Laboratory of Resource Chemistry of Ministry of Education, 100 Guilin Road, Shanghai 200234, People’s Republic of China
linked immunosorbent assay (ELISA) [8], have been explored. Although such methods have good sensitivity and selectivity, they require tedious sample pretreatment, and well training operators. In addition, some sensors have been developed to analyze CAP. For instance, Yin et al. [9] reported a sensing strategy by using aptamer-based photoelectrochemistry for determination of CAP, but still time-consuming. Hong et al. [10] developed an enzyme-free fluorometri
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