Fabrication of Non-enzymatic Electrochemical Glucose Sensor Based on Nano-copper Oxide Micro Hollow-spheres
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pISSN 1226-8372 eISSN 1976-3816
RESEARCH PAPER
Fabrication of Non-enzymatic Electrochemical Glucose Sensor Based on Nano-copper Oxide Micro Hollow-spheres Zahra Haghparas, Zoheir Kordrostami, Mohsen Sorouri, Maryam Rajabzadeh, and Reza Khalifeh
Received: 24 February 2020 / Revised: 14 June 2020 / Accepted: 14 June 2020 © The Korean Society for Biotechnology and Bioengineering and Springer 2020
Abstract In the present study, an electrochemical glucose biosensor has been developed based on nano-copper oxide micro hollow spheres. The nano-copper oxide micro hollow spheres were synthesized via hydrothermal method using pluronic F-127 as a surfactant. For structural characterization of CuO hollow sphere structures, the scanning electron microscopy and X-ray diffraction spectroscopy were applied. The performance parameters of the sensor were improved by optimizing the modification process. The electrochemical characteristics of the proposed glucose biosensor were investigated by using cyclic voltammetry and chronoamperometry techniques in both 0.1 M and 1 M NaOH solutions. The results revealed that the proposed electrode has a wide dynamic range from 1 μM to 11.50 mM for glucose detection at 0.1 M NaOH solution which covers two linear ranges from 1 μM to 3 mM and from 3 mM to 11.50 mM. The sensitivities of the two linear ranges were obtained as 25.0 ± 0.8 μA·mM-1·cm-2 and 13.6 ± 0.3 μA·mM-1·cm-2, respectively. An extremely wide linear range from 1 µM to 16 mM with a sensitivity of 35.2 ± 0.4 μA·mM-1·cm-2 was achieved for the 1 M NaOH solution. The sensor achieved a 1 μM practical lowest limit of detection which is an excellent low limit of detection at both NaOH concentrations compared to some important previously reported works. In addition, the good tolerance toward the interfering species and the satisfactory behavior Zahra Haghparas, Zoheir Kordrostami* Department of Electrical and Electronic Engineering, Shiraz University of Technology, Shiraz, Iran Tel/Fax: +98-71-37354500 E-mail: [email protected] Mohsen Sorouri*, Maryam Rajabzadeh, Reza Khalifeh Department of Chemistry, Shiraz University of Technology, Shiraz, Iran Tel/Fax: +98-71-37354500 E-mail: [email protected]
in real sample analysis verified the promising performance of the proposed sensor. Keywords: nano-copper oxide, micro hollow spheres, hollow spheres, glucose sensor, electrochemical sensor
1. Introduction Diabetes mellitus is a metabolic disorder in the body that is characterized by high blood sugar and insulin resistance [1,2]. According to the World Health Organization (WHO) report, the number of diabetics will reach 642 million by 2040 [1,3]. Careful control of diabetes and normalization of the blood sugar in diabetic patients can prevent longterm adverse effects and guarantee the normal life of patients [1,2,4,5]. The first biosensor, a glucose oxidasebased enzymatic electrode, was developed by Leland Clark in early 1962 [6,7]. Afterward, hundreds of biosensors have been built in many research laboratories around the world [8-12]. T
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