Fabrication of a sensitive and selective electrochemical sensing platform based on poly- l -leucine modified sensor for
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ORIGINAL PAPER
Fabrication of a sensitive and selective electrochemical sensing platform based on poly‑l‑leucine modified sensor for enhanced voltammetric determination of Riboflavin Amrutha Balliamada Monnappa1,2 · Jamballi Gangadharappa Gowda Manjunatha1 · Aarti Sripathi Bhatt2 · Pushpanjali Pemmatte Ananda1 Received: 15 June 2020 / Accepted: 17 August 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Riboflavin (RB) plays a pivotal role in cell growth and various biochemical reactions in the human body. Low level of RB leads to various health related issues and hence it is imperative to develop a sensitive and selective method for determination of RB. Electrochemical property of RB at poly-l-leucine modified carbon paste electrode (PLMCPE) was examined by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) in 0.2 M phosphate buffer solution (PBS) of an optimum pH of 6.5. Favourable interaction between l-leucine (l-leu) film and RB enhance the redox current response in comparison with bare carbon paste electrode (BCPE). The process at the exterior of the electrode was noticed to be reversible and diffusion controlled in the potential window of − 0.8 to 0 V/s. The surface characterisation of fabricated PLMCPE was accomplished by Field emission scanning electron microscopy (FE-SEM) and Electronic impedance spectroscopy (EIS). Parameters like electrochemical active surface area of the sensor, number of electrons transferred, surface concentration and heterogeneous rate constant were calculated. Under optimised condition DPV and CV divulge a notable linear relationship in between the oxidation peak current of RB and concentration of RB with a detection limit (LOD) of 3.4 × 10–8 M and 4.02 × 10–8 M respectively. Various interferant metal ions were used to investigate the interference effect and was found that the method is compelling, selective and sensitive towards detection of RB. The performance of PLMCPE was triumphantly tested for the detection of RB in vitamin capsules by DPV and the excellent recovery proves that the proposed method can be constructively used for real sample analysis. Keywords Carbon paste electrode · Poly-l-leucine · Modified sensor · Riboflavin · Vanillin
Introduction RB or Vitamin B 2 is a water-soluble vitamin which plays a crucial role in various biochemical reactions of human body like haemoglobin synthesis, drugs and xenobiotics, hydrogen carrier in the course of carbohydrate, protein and lipid metabolism [1]. It is a vital component of flavoenzymes which displays a significant role in the flavoprotein enzyme * Jamballi Gangadharappa Gowda Manjunatha [email protected] 1
Department of Chemistry, FMKMC College, Madikeri, Constituent College of Mangalore University, Mangalore, Karnataka, India
Department of Chemistry, N.M.A.M. Institute of Technology, (Visvesvaraya Technological University, Belgavi) Nitte, Udupi District, Karnataka 574110, India
2
reaction and plays a pivotal role as component of electron transport system in
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