G Protein-Coupled Receptor Signaling Methods and Protocols
This detailed volume assembles comprehensive protocols to assist with the study of structural, molecular, cell biological, and in vivo facets of GPCRs, and to enable the development of experimental tools for screening novel GPCR drugs. Sections explore th
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Mario Tiberi Editor
G Protein-Coupled Receptor Signaling Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
G Protein-Coupled Receptor Signaling Methods and Protocols
Edited by
Mario Tiberi Ottawa Hospital Research Institute (Neurosciences), Ottawa, ON, Canada Department of Cellular and Molecular Medicine, University of Ottawa Brain and Mind Research Institute, University of Ottawa, Ottawa, ON, Canada
Editor Mario Tiberi Ottawa Hospital Research Institute (Neurosciences) Ottawa, ON, Canada Department of Cellular and Molecular Medicine University of Ottawa Brain and Mind Research Institute University of Ottawa Ottawa, ON, Canada
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9120-4 ISBN 978-1-4939-9121-1 (eBook) https://doi.org/10.1007/978-1-4939-9121-1 Library of Congress Control Number: 2019932813 © Springer Science+Business Media, LLC, part of Springer Nature 2019 Chapter 4 is licensed under the terms of the Creative Commons Attribution 4.0 International License (http:// creativecommons.org/licenses/by/4.0/). For further details see license information in the chapter. This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Cover Art: Genevie`ve Laroche and Patrick Gigue`re This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface G protein-coupled receptors (GPCRs) represent the largest transmembrane protein families in the proteome of most living organisms. They have evolved as key communication players by virtue of sensing extracellular cues such as light, odorants,
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