Genome sequence of a spore-laccase forming, BPA-degrading Bacillus sp. GZB isolated from an electronic-waste recycling s
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ORIGINALPAPER
Genome sequence of a spore-laccase forming, BPA-degrading Bacillus sp. GZB isolated from an electronic-waste recycling site reveals insights into BPA degradation pathways Ranjit Das1,2 · Zhishu Liang1,2 · Guiying Li2 · Bixian Mai1 · Taicheng An2 Received: 10 September 2018 / Revised: 18 January 2019 / Accepted: 22 January 2019 © Springer-Verlag GmbH Germany, part of Springer Nature 2019
Abstract Bisphenol A (BPA) is a synthetic chemical with known deleterious effects on biota. A genome sequencing project is an important starting point for designing a suitable BPA bioremediation process, because it provides valuable genomic information about the physiological, metabolic, and genetic potential of the microbes used for the treatment. This study explored genomic insights provided by the BPA-degrading strain Bacillus sp. GZB, previously isolated from electronic-waste-dismantling site. The GZB genome is a circular chromosome, comprised of a total of 4,077,007 bp with G+C content comprising 46.2%. Genome contained 23 contigs encoded by 3881 protein-coding genes with nine rRNA and 53 tRNA genes. A comparative study demonstrated that strain GZB bloomed with some potential features as compared to other Bacillus species. In addition, strain GZB developed spore cells and displayed laccase activity while growing at elevated stress levels. Most importantly, strain GZB contained many protein-coding genes associated with BPA degradation, as well as the degradation of several other compounds. The protein-coding genes in the genome revealed the genetic mechanisms associated with the BPA degradation by strain GZB. This study predicts four possible degradation pathways for BPA, contributing to the possible use of strain GZB to remediate different polluted environments in the future. Keywords Bisphenol A · Bioremediation · Bacillus sp. GZB · Electronic-waste · Genome
Introduction
Communicated by Erko Stackebrandt. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-019-01622-2) contains supplementary material, which is available to authorized users. * Guiying Li [email protected] 1
State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China
Guangzhou Key Laboratory Environmental Catalysis and Pollution Control, Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control, School of Environmental Science and Engineering, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou 510006, China
2
Bisphenol A [BPA; 2, 2-bis (4-hydroxyphenyl) propane] is a synthetic organic compound, produced at high volumes worldwide (Huang et al. 2012; Im et al. 2016). BPA is an essential constituent in the manufacturing of polycarbonate plastics, epoxy resins, and other applied materials; these materials are found in many every day products, such as baby bottles, food containers, thermal papers, lacquers, water pipes, toys, medical equi
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