Hepatocytes Methods and Protocols
Hepatocytes account for approximately 80% of the liver mass and play a significant role in various aspects of liver physiopathology, exhibiting unrivaled complexity and diversity of functions. In Hepatocytes: Methods and Protocols, expert researchers prov
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MO L E C U L A R BI O L O G Y
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For other titles published in this series, go to www.springer.com/series/7651
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Hepatocytes Methods and Protocols
Edited by
Patrick Maurel INSERM, Université Montpellier 1, and Institut de Recherche en Biothérapie, Montpellier, France
Editor Patrick Maurel INSERM, Université Montpellier 1, and Institut de Recherche en Biothérapie INSERM U632 Physiopathologie Hépatique 1919 rte de Mende 34293 Montpellier France [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-60761-687-0 e-ISBN 978-1-60761-688-7 DOI 10.1007/978-1-60761-688-7 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2010921223 © Springer Science+Business Media, LLC 2010 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Cover illustration: Expression and distribution of cytoskeletal elements and cell adhesion molecules in primary cultures of human hepatocytes maintained in a sandwich culture configuration. Immunofluorescent images showing in vivo-like distribution of actin microfilaments (upper left panel), microtubules (upper right panel), E-cadherin (lower left panel) and gap junctions (Cx-32) (lower right panel). Phenotypic expression of the cytoskeleton and cell adhesion proteins in vitro is determined, in part, by the capacity of the cells to form cell-cell contacts and intercellular communications. For more information on the isolation and culture of primary human hepatocytes see Chapter 3. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Foreword Primary human hepatocytes offer a unique model for investigating basic mechanisms of tissue differentiation, proliferation, and viability as well as a privileged tool for testing drug metabolism, efficacy, and toxicity. Thus, dissecting the extracellular and intracellular signals which drive primary hepatocyte biology
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