Lymphangiogenesis Methods and Protocols
This volume discusses the latest tools, techniques, and animal models designed to study the processes of lymphatic vascular formation in vivo and in vitro and its functions in health and disease. The chapters in the book cover topics such as genetics line
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Guillermo Oliver Mark L. Kahn Editors
Lymphangiogenesis Methods and Protocols
Methods
in
M o l e c u l a r B i o lo g y
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Lymphangiogenesis Methods and Protocols
Edited by
Guillermo Oliver Center for Vascular & Developmental Biology, Feinberg Cardiovascular Research Institute, Northwestern University, Chicago, IL, USA
Mark L. Kahn Department of Medicine and Cardiovascular Institute, University of Pennsylvania, Philadelphia, PA, USA
Editors Guillermo Oliver Center for Vascular & Developmental Biology Feinberg Cardiovascular Research Institute Northwestern University Chicago, IL, USA
Mark L. Kahn Department of Medicine and Cardiovascular Institute University of Pennsylvania Philadelphia, PA, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-8711-5 ISBN 978-1-4939-8712-2 (eBook) https://doi.org/10.1007/978-1-4939-8712-2 Library of Congress Control Number: 2018953044 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface All animals with a blood vascular system also have a lymphatic vascular system. The lymphatic vasculature is essential for maintaining fluid homeostasis, immune surveillance, and fat uptake from the intestinal tract. The lymphatic vasculature forms a complex, hierarchical network that contains two morphologically different types of vessels with distinct functions. Blind-ended initial lymphatics with discontinuous button-like
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