Maize Methods and Protocols
This volume discusses techniques used for the molecular characterization of maize. This book is divided into 4 parts: cell, tissue, and organ culture and maize transformation; gene silencing and generation of mutant populations; plant gene expression; and
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L. Mark Lagrimini Editor
Maize Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences, University of Hertfordshire, Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Maize Methods and Protocols
Edited by
L. Mark Lagrimini Department of Agronomy and Horticulture, University of Nebraska – Lincoln, Lincoln, NE, USA
Editor L. Mark Lagrimini Department of Agronomy and Horticulture University of Nebraska – Lincoln Lincoln, NE, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7314-9 ISBN 978-1-4939-7315-6 (eBook) DOI 10.1007/978-1-4939-7315-6 Library of Congress Control Number: 2017952059 © Springer Science+Business Media LLC 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media, LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface More maize is grown more than any other food crop worldwide with production at nearly 1 billion metric tons/year. In the USA, corn yield has increased at a steady 1.6 bushels/ acre/year since the 1930s, realized through combined genetic and crop management improvements. The demand for greater yields and more sustainable production will increase dramatically as population increases, the amount of arable land shrinks from urban sprawl and salinization, and the threat from biotic and abiotic stressors increases as a consequence of climate change. Looking ahead, the theoretical yield potential for maize is estimated at 300% of that which is achieved in today’s best hybrids, making this crop a fruitful subject for molecular biology studies and genetic improvement. Unlike laboratory models such as Arabido
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