In vitro and in silico studies of the antifungal properties of the bulb and leaves extracts of Drimia delagoensis Baker
- PDF / 1,600,931 Bytes
- 7 Pages / 595.276 x 790.866 pts Page_size
- 48 Downloads / 190 Views
RESEARCH ARTICLE
In vitro and in silico studies of the antifungal properties of the bulb and leaves extracts of Drimia delagoensis Baker (Jessop) Nokuthula Ndaba1 · Marthe Carine Fotsing1 · William Wilson Anku1 · Penny Poomani Govender1 Received: 22 October 2019 / Accepted: 26 November 2019 © Institute of Korean Medicine, Kyung Hee University 2019
Abstract Drimia delagoensis (D. delagoensis), a member of the Hyacinthaceae family, is commonly used for the treatment of heartwater and skin ailments by the Zulus in South Africa. In this research study, the bulb and leaves of D. delagoensis were tested for their antifungal efficacy against Aspergillus fumigatus. Eight crude extracts were further analysed to determine the volatile chemical components of the plant using one-dimensional gas chromatography coupled with the time of fight mass spectrometry. The compounds detected from GC-MS were further subjected to molecular docking study to determine prospective relationship between the analysed compounds (1–9) and the active site of the glucosamine-6-phosphate synthase. All the crude extracts were effective against A. fumigatus. However, it was observed that dichloromethane: methanol (1:1) mixture for the bulb extracts had a high minimum fungal concentration values of (7.8 µg/mL) followed by hexane (7.8 µg/ mL) and ethyl acetate (7.8 µg/mL). Compounds (9Z,12Z)-9,12-Octadecadienoic acid(R)-2,3-bis[[(E)-2-methyl-2-butenoyl] oxy]propyl ester and 9-Octadecenoic acid (9Z)-,1-methyl-1,2-ethanediyl ester had higher binding affinities of − 47.69 and − 62.36 kcal/mol, respectively when compared to amphotericin B standard drug (− 44.19 kcal/mol). Furthermore, lipophilicity values for ligands, which ranged from − 11.46 to − 27.07, were more significant than those for amphotericin B (− 19.66), and this greater significance could also be ascribed to the potency of the crude extracts against A. fumigatus. Keywords Aspergillus fumigatus · Molecular docking · Plant extract · Microbroth dilution · Antifungal activity · Drimia delagoensis (Jessop) Baker
Introduction Globally, fungi mycotoxins have been found in animal, animal feeds/plants products, and in the air, and they have become a significant threat to agriculture and human health. The secondary metabolites of Fusarium, Aspergillus and Penicillium fungus, which are often isolates from cereals, such as rice, soybeans and wheat, find their way into humans through direct consumption or persons coming into contact with decomposed animal/plant material (Kovačević et al. 2014). Tryptoquivaline, Fumagillin, Fumitoxin, helvolic acid, monotrypacidin, fumigatin and among others are Aspergillus fumigatus (A. fumigatus) secondary metabolite toxins with immunosuppressive properties that * Penny Poomani Govender [email protected] 1
Department of Chemical Sciences, Faculty of Science, University of Johannesburg, Doornfontein Campus, P. O. Box 17011, Johannesburg 2028, South Africa
initiate apoptosis in cells of the immune system. Aspergillus fumigatus is an airborne pathogen present in both
Data Loading...
