Isolation and characterization of 25 microsatellite loci from the Pacific bluefin tuna Thunnus orientalis (Perciformes,
- PDF / 183,223 Bytes
- 3 Pages / 595.276 x 790.866 pts Page_size
- 109 Downloads / 201 Views
MICROSATELLITE LETTERS
Isolation and characterization of 25 microsatellite loci from the Pacific bluefin tuna Thunnus orientalis (Perciformes, Scombridae) Naoki Yagishita • Yoshifumi Sawada Yasuo Agawa • Toru Kobayashi
•
Received: 23 August 2013 / Accepted: 23 September 2013 / Published online: 29 September 2013 Ó Springer Science+Business Media Dordrecht 2013
Abstract The pacific bluefin tuna (PBT) Thunnus orientalis is one of the most important species for fisheries in the world. This species has been highly exploited in fisheries, resulting in dwindling of its natural resources. The artificial hatching of a third generation of fully cultured PBT was a success in 2007, suggesting the possibility of mass production of PBT for food and for seed release. To enhance stock structure studies and investigate genetic feature of seedlings, we isolated 25 polymorphic microsatellite loci (2–18 alleles/locus; expected heterozygosity, 0.042–0.941) from PBT. Keywords Thunnus orientalis Pacific bluefin tuna Microsatellites PCR primers
The pacific bluefin tuna (PBT) Thunnus orientalis is distributed widely in the North Pacific Ocean, and one of the most important species for fisheries in the world. This species has been highly exploited in commercial fisheries, resulting in dwindling of its natural resources (The Ministry of Agriculture, Forestry and Fisheries of Japan 2007). The spawning grounds of PBT have been suggested to be formed in the north-western Pacific Ocean and the Sea of Japan, based on the occurrence of the larvae and the dates of hatching of juveniles (Tanaka et al. 2007). However, it is N. Yagishita (&) T. Kobayashi Department of Fisheries, Graduate School of Agriculture, Kinki University, 3327-204 Naka-machi, Nara 631-8505, Japan e-mail: [email protected] Y. Sawada Y. Agawa Ohshima Experiment Station, The Fisheries Laboratory, Kinki University, 1790-4 Oshima, Kushimoto-cho, Wakayama 649-3633, Japan
not clear whether these spawning grounds comprise one or more stocks, and the geographical structure of wild populations of the species is still unknown. Since the whole life cycle of PBT under aquaculture conditions was completed in 2002 (Sawada et al. 2005), technology for cultivation of PBT has advanced considerably and artificial hatching of a third generation of fully cultured PBT was a success in 2007. This success suggests the possibility of mass production of PBT for food and for seed release. Although a total of 27 microsatellite markers have been developed (Takagi et al. 1999; Morishima et al. 2009), more microsatellites are required to enhance stock structure studies and to investigate genetic feature of seedlings. Therefore, we developed and characterized 25 new microsatellites markers in PBT. Microsatellite markers were developed based on 575 end-sequence reads from clones of a BAC library of PBT stored in our laboratory. Among the sequence reads, we identified microsatellite loci with more than five repeats of a unit consisting of at least two nucleotides. Primers for amplifying each
Data Loading...
