Isolation and characterization of 12 microsatellite loci from the endemic Schizothorax chongi (Fang, 1936)
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MICROSATELLITE LETTERS
Isolation and characterization of 12 microsatellite loci from the endemic Schizothorax chongi (Fang, 1936) Yanfu Que • Zhiqing Ren • Zhi Yang • Huiyuan Tang • Shaobo Gao • Weitao Li
Received: 1 December 2013 / Accepted: 25 December 2013 Ó Springer Science+Business Media Dordrecht 2013
Abstract Schizothorax chongi is an endemic and important polyploidy fish in the upper reaches of the Yangtze River. S. chongi represents a typical model species to study historical adaptation and evolution in the Tibetan Plateau. Twelve microsatellite markers were developed and eleven loci showed polymorphism while only one (SC11) showed monomorphism. The number of observed alleles ranged from 2 to 9, and the mean expected heterozygosity (HE) and Shannon–Wiener Diversity Indices (H0 ) of all 11 polymorphic loci ranged from 0.061 to 0.854, from 0.138 to 2.043, respectively. These microsatellite markers will provide helpful tools not only for genetic analyses of wild S. chongi but also for possible re-stocking management in the long turn. Keywords Schizothorax chongi Endemic fish Microsatellite loci FIASCO Genetic structure
Schizothorax chongi (Fang, 1936) (Cypriniformes: Cyprinidae: Schizothoracinae), which distributes in the upper stream of the Yangtze River and its tributaries, is an endemic cold-water fish species (Ding 1994). It is one of an important model species of subfamily Schizothoracinae to study adaptation and evolution of polyploidy fish associated with the uplift of the Tibetan Plateau (Zan et al. 1985). And the species is also one of the dominant commercial
Y. Que Z. Ren Z. Yang H. Tang S. Gao W. Li (&) Key Laboratory of Ecological Impacts of Hydraulic-Projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources, Institute of Hydroecology, Ministry of Water Resources and Chinese Academy of Sciences, Wuhan 430079, People’s Republic of China e-mail: [email protected]
species in the Yalong River in southwestern China, which is one of the main tributaries in the Yangtze River. However, wild population of S. chongi declined dramatically in recent decade due to over-fishing, water pollution and hydro-power constructions (Zhu and Chang 2008). Protecting wild Schizothorax now has been appraised as critical by our government. To better address conservation plan of this endemic species, background of genetic structure is essential for protecting S. chongi wild population. However, no suitable nuclear DNA markers have been available for the species. Microsatellites are considered as a powerful genetic marker for population genetic, and are of great utility in species conservation and management. Here we first developed twelve microsatellite loci from S. chongi for the purpose of protecting its wild population. Total genomic DNA was extracted from the fin tissues using a proteinase K/phenol–chloroform method. Enriched partial microsatellite genomic libraries for repeat motifs (GATA)8, and (GT)15 were obtained, following essentially the FIASCO protocol with the a few mod
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