Isolation and characterization of microsatellite loci from Iguana delicatissima ( Reptilia: Iguanidae ), new perspective
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TECHNICAL NOTE
Isolation and characterization of microsatellite loci from Iguana delicatissima (Reptilia: Iguanidae), new perspectives for investigation of hybridization events with Iguana iguana V. Valette • L. Filipova´ • B. Vuillaume • C. Cherbonnel • A. M. Risterucci • C. Delaunay M. Breuil • F. Grandjean
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Received: 17 August 2012 / Accepted: 22 August 2012 / Published online: 6 September 2012 Ó Springer Science+Business Media B.V. 2012
Abstract A (GA)n and (GT)n microsatellite-enriched library was constructed and 25 nuclear simple sequence repeat (SSR) loci were characterized in the Lesser Antillean Iguana (Iguana delicatissima). All SSR loci were found to be polymorphic after screening for diversity in different cultivars, and a cross-taxa amplification tests showed the potential transferability of most SSR markers in Iguana iguana. First to be published for I. delicatissima, this new SSR resource will be a powerful tool for intraspecific genetic studies and for investigation of hybridization events with Iguana iguana. Keywords Iguana delicatissima Iguana iguana Microsatellite
Lesser Antillean Iguana (Iguana delicatissima) is endemic to the Lesser Antilles, inhabiting this archipelago from Martinique to Anguilla. In Guadeloupean Archipelago V. Valette C. Cherbonnel Genindexe, 17000 La Rochelle, France L. Filipova´ C. Delaunay F. Grandjean (&) Laboratoire Ecologie, Evolution, Symbiose, UMR CNRS 6556, Universite´ de Poitiers, 40, av. du Recteur Pineau, 86022 Poitiers Cedex, France e-mail: [email protected] B. Vuillaume M. Breuil De´partement de Syste´matique et d’E´volution, AmphibiensReptiles, Muse´um national d’Histoire naturelle, 57, rue Cuvier, CP no 30, 75231 Paris Cedex 05, France A. M. Risterucci CIRAD (Centre de coope´ration Internationale en Recherche Agronomique pour le De´veloppement), UMR 108 AGAP, TA A 108/03 Avenue Agropolis, 34398 Montpellier Cedex 5, France
hybridization detected on a morphological basis between the common or green Iguana (Iguana iguana) and (I. delicatissima) have lead to the elimination of the endemic species from four islands (Basse-Terre, Grande-Terre, Terre-de-Haut and Terre-de-Bas des Saintes) (Breuil 2002). Because this situation will become worst, Iguana Specialist Group upgraded I. delicatissima from vulnerable to endangered (Breuil et al. 2010). In order to know what impacts could have this invasion, it is important to monitor genetic evolution in different populations of I. delicatissima. Several studies have shown that microsatellites are good tools to monitor genetic evolution of populations (Roy et al. 1994) but, to date, no microsatellite was described in the Iguana genus. In this study, we characterized 25 microsatellites in I. delicatissima and we tested them by cross-species amplifications on a population of I. iguana. We extracted I. delicatissima genomic DNA from blood samples of an individual captured in Chancel, Martinique, using the Generation DNA purification Capture Plate Kit (QIAGEN). A microsatellite-enriched library
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