LC-MS/MS in Proteomics Methods and Applications
With the development of new quantitative strategies and powerful bioinformatics tools to cope with the analysis of the large amounts of data generated in proteomics experiments, liquid chromatography with tandem mass spectrometry (LC-MS/MS) is making poss
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MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For other titles published in this series, go to www.springer.com/series/7651
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LC-MS/MS in Proteomics Methods and Applications
Edited by
Pedro R. Cutillas Centre for Cell Signalling, Institute of Cancer, Bart’s and the London School of Medicine, Queen Mary, University of London, UK
John F. Timms Cancer Proteomics Group, EGA Institute for Women’s Health, University College London, London, UK
Editors Pedro R. Cutillas Institute of Cancer Centre for Cell Signalling Bart’s and the London School of Medicine Queen Mary, Universtiy of London Charterhouse Square EC1M 6BQ London 3rd Floor John Vane Science Centre London, UK [email protected]
John F. Timms Cancer Proteomics Group EGA Institute for Women’s Health University College London Gower Street WC1E 6BT London Cruciform Building 1.1.09 London, UK [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-60761-779-2 e-ISBN 978-1-60761-780-8 DOI 10.1007/978-1-60761-780-8 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2010930850 © Springer Science+Business Media, LLC 2010 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface The aim of this book is to be a useful resource for experienced proteomics practitioners as well as an aid to newcomers in order to become acquainted with the theory and practice of a wide array of mass spectrometric techniques for proteome research. The role of mass spectrometry (MS) in proteomics has gradually evolved from being a technique used to identify proteins in gels to be the preferred method for protein detection and quantification. Seminal work at the beginning of this century demonstrated that the hyphenation of liquid chromatography with tandem mass spectrometry (LC-MS/MS) allowed the protein biochemist to quantify thousands of proteins within time frames that make these techniques powerful readouts for biological experiments. Consequently, LC-MS/MS has found a niche in many different areas of biological and biomedical research, mainly due to the introduction of user-friendly and high-performance instrumentation and because of the development of new
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