Histochemistry of Single Molecules Methods and Protocols
This detailed volume explores numerous histochemical techniques through a series of lab-tested protocols for the detection of specific molecules or metabolic processes, both at light and electron microscopy. More in detail, the book is divided into six se
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Carlo Pellicciari Marco Biggiogera Editors
Histochemistry of Single Molecules Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Histochemistry of Single Molecules Methods and Protocols
Edited by
Carlo Pellicciari and Marco Biggiogera Department of Biology and Biotechnology, University of Pavia, Pavia, Italy
Editors Carlo Pellicciari Department of Biology and Biotechnology University of Pavia Pavia, Italy
Marco Biggiogera Department of Biology and Biotechnology University of Pavia Pavia, Italy
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6787-2 ISBN 978-1-4939-6788-9 (eBook) DOI 10.1007/978-1-4939-6788-9 Library of Congress Control Number: 2016962655 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Cover Illustration: Double staining of claudin-5 (red fluorescence) and beta-actin (green) in the glomerulus of mouse kidney in a formalin-fixed paraffin-embedded tissue. Beta-actin and claudin-5 colocalize along the capillary walls showing yellow to orange color, which might correspond to pedicels of podocytes. Beta-actin is also demonstrated in the mesangial cells. Nuclei were stained with the DNA-specific dye DAPI (blue fluorescence). (Courtesy of Shuji Yamashita and Osamu Katsumata.) Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface The story of histochemistry goes a long way back and, in the last 150 years, has evolved in parallel with the operating instruments, from bright field microscopy to super-resolution microscopy. In this evolution resides the uniqueness of this science. Compared to the biochemical and molecul
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