Overexpression of HOXA-AS2 LncRNA in Patients with Gastric Cancer and Its Association with Helicobacter pylori Infection
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ORIGINAL RESEARCH
Overexpression of HOXA-AS2 LncRNA in Patients with Gastric Cancer and Its Association with Helicobacter pylori Infection Ali Rajabi 1 & Atousa Riahi 1 & Hanie Shirabadi-Arani 1 & Yaghoub Moaddab 2 & Mehdi Haghi 1 & Reza Safaralizadeh 1 Accepted: 2 November 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Purpose LncRNAs are regulatory factors that play a prominent role in the carcinogenesis processes and cancer cell ability to invade and metastasize. Hence, lncRNAs are considered as the potential diagnostic and therapeutic biomarkers in diverse malignancies. The present study was designed to assess the difference of HOXA-AS2 gene expression levels in cancerous tissues as compared to marginal noncancerous tissues of gastric cancer patients. Methods Fifty pairs of cancerous and marginal noncancerous tissue of gastric cancer patients were collected in the present study. Then, RNA extraction and cDNA synthesis were performed for all specimens. The qRT-PCR was carried out to examine the difference of HOXA-AS2 gene expression. Furthermore, the association between HOXA-AS2 expression and the clinicopathological features as well as the function of HOXA-AS2 biomarkers was evaluated. Results The HOXA-AS2 expression was significantly elevated in cancerous tissues as compared to marginal noncancerous tissues in gastric cancer patients (p < 0.0001). Analysis of gene expression data revealed that there was a significant association between an increased HOXA-AS2 gene expression and clinicopathological features such as tumor size ˃ 5 cm (p = 0.009), lymph node metastasis (p = 0.028), and H. pylori infection (p = 0.011). The results of ROC analysis indicated that HOXA-AS2 with AUC, sensitivity, and specificity of 0.816, 92%, and 70%, respectively, can act as a potential biomarker (CI 95% = 0.7297– 0.9023). Conclusion With regard to the overexpression of HOXA-AS2 in gastric cancer tissues, the mentioned gene may serve as an oncogenic lncRNA in gastric cancer patients. Moreover, HOXA-AS2 can act as a potential biomarker in molecular targeted therapies to recognize and treat gastric cancer patients. Keywords Gastric cancer . lincRNA HOXA-AS2 . qRT-PCR
Introduction Gastric cancer (GC) with the highest mortality, morbidity, and recurrence rates is the third deadly malignancy in the world following lung and colorectal cancers. According to GLOBOCAN reports in 2018, about 800,000 deaths occurred due to this cancer [1]. A variety of genetic and environmental risk factors are implicated in the emergence and progression of GC. Infection with H. pylori as a group I carcinogen with * Reza Safaralizadeh [email protected] 1
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
2
Liver and Gastroenterology Disease Research Center, Tabriz University of Medical Science, Tabriz, Iran
diverse virulence elements such as CagA or VacA is one of the most influential environmental factors involved in GC development. In recent decades, although adva
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