Overexpression of MdPHR1 Enhanced Tolerance to Phosphorus Deficiency by Increasing MdPAP10 Transcription in Apple ( Malu
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Overexpression of MdPHR1 Enhanced Tolerance to Phosphorus Deficiency by Increasing MdPAP10 Transcription in Apple (Malus × Domestica) Rui Li1,2 · Jian‑Ping An1 · Chun‑Xiang You1 · Xiao‑Fei Wang1 · Yu‑Jin Hao1 Received: 26 June 2019 / Accepted: 11 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Phosphorus is an essential nutrient during plant growth and development. It is involved in the formation of important compounds in plants, such as phospholipids, ATP, and nucleic acids. However, phosphorus is distributed unevenly in most soils, and exists primarily in the form of organophosphorus, which plants cannot use. In this study, the AtPHR1 homolog MdPHR1 was identified in apple. Our results showed that MdPHR1 contained a MYB domain and a coiled-coil domain, which were conserved in AtPHR1. The MdPHR1 transgenic lines had higher acid phosphatase activity and phosphorus content than the wild-type under phosphorus limited conditions. Overexpression of MdPHR1 enhanced tolerance to phosphorus deficiency in apple calli and Arabidopsis seedlings, and MdPHR1 could bind to the P1BS cis-element of MdPAP10 and activated its transcription. Collectively, our experimental evidence suggests that MdPHR1 may be the central regulator of the system controlling transcriptional responses to Pi starvation in apple. Keywords MdPHR1 · Pi starvation · Acid phosphatase activity · Apple
Introduction The growth and development of plants largely depends on the supply of mineral nutrients available in the soil. Phosphorus is one of the essential elements for plant growth, as it is the main component of nucleic acids, phospholipids, adenosine phosphate in plants. Phosphorus is also an indispensable nutrient for plants to maintain life activities, energy transfer, and metabolism (Marschner and Rimmington Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00344-020-10225-x) contains supplementary material, which is available to authorized users. * Xiao‑Fei Wang [email protected] * Yu‑Jin Hao [email protected] 1
National Key Laboratory of Crop Biology, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, Shandong Agricultural University, Tai’an 271018, People’s Republic of China
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, People’s Republic of China
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1988). However, most phosphorus in soil exists in the form of organic phosphorus, which is difficult for plants to directly absorb and utilize (Bieleski 1973). Due to their immobility, plants often face significant challenges in absorbing sufficient phosphorus to meet their basic cellular needs. Therefore, plants have evolved a number of strategies to deal with limited phosphorus conditions, including a low phosphorus response mechanism and morphological, developmental, and physiological changes during the long course of evolution (Nilsson et al. 2007). Transcription factors, su
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