Overexpression of miR-27a predicts poor prognosis and promotes the progression in cholangiocarcinoma
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ORIGINAL ARTICLE
Overexpression of miR‑27a predicts poor prognosis and promotes the progression in cholangiocarcinoma Yunxia Liu1 · Xia Liu2 · Yanhua Zhou3 · Tingting Liu1 · Jie Li1 Received: 23 February 2020 / Accepted: 13 August 2020 © Springer Nature Switzerland AG 2020
Abstract The function of microRNA-27a (miR-27a) expression in cholangiocarcinoma (CCA) remains largely unclear; therefore, this study aimed to investigate the clinical significance and functional role of miR-27a in CCA. This study included 117 paired CCA tissues and adjacent normal tissues from CCA patients who received surgical resection. Reverse transcriptionquantitative polymerase chain reaction was used to measure the expression levels of miR-27a in CCA tissues and cell lines. A Kaplan–Meier curve and Cox regression analysis were used to determine overall prognostic performance. The effects of miR-27a on cell proliferation, migration, and invasion were measured by CCK-8 and Transwell assays. The expression levels of miR-27a in patients with CCA and cell lines were higher than those in adjacent normal tissues and normal cells, respectively. Additionally, miR-27a levels were found to be associated with lymph node metastasis and TNM stages. The overall survival time of CCA patients with high miR-27a expression was poorer than that of those with low miR-27a expression. Furthermore, miR-27a overexpression promoted CCA cell proliferation, migration, and invasion, whereas knockdown of miR-27a suppressed cell proliferation, migration, and invasion. Taken together, these results suggest the potential usefulness of miR-27a in the prognosis and progression of CCA. Keywords MicroRNA-27a · Cholangiocarcinoma · Prognosis · Proliferation · Migration · Invasion Abbreviations CCA Cholangiocarcinoma qRT-PCR Reverse transcription-quantitative polymerase chain reaction miRNAs MicroRNAs HIBEpic Human intrahepatic bile duct epithelial cell line NCs Negative controls cDNA Complementary DNA CCK-8 Cell counting kit-8 HR Hazard ratio * Yunxia Liu [email protected] 1
Department of Internal Medicine, Fuyanshan Branch of Affiliated Hospital of Weifang Medical University, Intersection of Limin Road and Fuyuan Street, Weifang 261053, Shandong, China
2
Department of Ophthalmology, Affiliated Hospital of Weifang Medical University, Weifang 261031, Shandong, China
3
Department of Oncology, Affiliated Hospital of Weifang Medical University, Weifang 261031, Shandong, China
CI Confidence interval TNM Tumor–node–metastasis
Introduction Cholangiocarcinoma (CCA) is a highly aggressive malignant tumor that is difficult to diagnose and has a high mortality rate; it originates from the ductular epithelium of the biliary tree [1]. CCA is still common in Asia, but the incidence of CCA has significantly increased globally in recent years in countries, such as in Europe and North America [2]. Radical surgery is the only curative treatment for CCA; however, the survival rate of CCA patients is still limited due to early invasion and metastasis [1, 3]. Al
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