Over-Expression of a Transcription Factor Gene BoC3H4 Enhances Salt Stress Tolerance but Reduces Sclerotinia Stem Rot Di
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Over‑Expression of a Transcription Factor Gene BoC3H4 Enhances Salt Stress Tolerance but Reduces Sclerotinia Stem Rot Disease Resistance in Broccoli Ming Jiang1 · Lixiang Miao2 · Huijuan Zhang1 · Xin Zhu1 Received: 6 March 2019 / Accepted: 7 November 2019 © Springer Science+Business Media, LLC, part of Springer Nature 2019
Abstract C3H-type zinc finger genes play diverse roles in plant growth, development, and stress responses. In our present study, a C3H-type gene namely BoC3H4 was isolated from broccoli. BoC3H4 was 1629 bp in length encoding 542 amino acid residues. The deduced protein sequence contained two ankyrin repeats and two CCCH zinc finger motifs, and those motifs shared high identities with homologous sequences from other Cruciferae plants. The expression levels of BoC3H4 elevated when subjected to both salt stress and Sclerotinia sclerotiorum infection. Broccoli plants with constitutive expression of BoC3H4 demonstrated increased tolerance toward salinity stress, accompanied by a prominent accumulation of proline, and a remarkable decrease of chlorophyll loss, MDA, REC, as well as H 2O2 accumulation compared to WT plants. Moreover, over-expression of BoC3H4 in broccoli lines decreased resistance to S. sclerotiorum, and it could not induce the expression of BoPDF1.2 gene, the marker gene for JA/ET signaling pathway. Our study proposes that BoC3H4 acts as a positive regulator of plant tolerance to salinity stress and a negative regulator of resistance to necrotrophic pathogen S. sclerotiorum. Keywords Broccoli · Sclerotinia sclerotiorum · C3H zinc finger · Salinity stress Abbreviations SA Salicylic acid ET Ethylene MeJA Methyl jasmonate ABA Abscisic acid MDA Malonyldialdehyde NJ Neighbor-joining REC Relative electrical conductivity qRT-PCR Quantitative real-time polymerase chain reaction WT Wild-type CTAB Cetyltrimethylammonium bromide NBT Nitroblue tetrazolium DAB 3,3′-Diaminobenzidine PR Pathogenesis related TF Transcription factors * Ming Jiang [email protected] 1
College of Life Sciences, Taizhou University, 1139# Shifu Road, Jiaojiang, Zhejiang, People’s Republic of China
Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang, China
2
ZF Zinc finger ROS Reactive oxygen species
Introduction Broccoli (Brassica oleracea var. italica) belongs to the family Cruciferae and is a close relative of cabbage (B. oleracea), cauliflower (B. oleracea var. capitata), kale (B. oleracea var. acephala), kohlrabi (B. oleracea var. caulorapa), and brussels sprouts (B. oleracea var. gemmifera), and all are considered as economically important vegetable crops in many countries (Kushad et al. 1999; Zabaras et al. 2013). Broccoli is a health-promoting vegetable because it is rich in nutrition and anticancer agents, including fatty acids, amino acids, vitamins, soluble fiber, mineral nutrients, selenium, glucosinolates, and phenolic compounds (Mahn and Reyes 2012; Kumar and Srivastava 2015; Hwang and Lim 2015). Broccoli has been becoming a highly val
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