Serum/Plasma Proteomics Methods and Protocols
Blood science has become a cornerstone of multiple disciplines, including clinical chemistry, disease diagnosis, and therapeutic monitoring. Over the past decade, we have witnessed the advent of increasingly powerful proteomics technologies that all
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
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Serum/Plasma Proteomics Methods and Protocols
Edited by
Richard J. Simpson and David W. Greening Ludwig Institute for Cancer Research Ltd, Royal Melbourne Hospital, Parkville, Victoria, Australia
Editors Richard J. Simpson, Ph.D. Ludwig Institute for Cancer Research Ltd Royal Melbourne Hospital Parkville, Victoria Australia [email protected]
David W. Greening, Ph.D. Ludwig Institute for Cancer Research Ltd Royal Melbourne Hospital Parkville, Victoria Australia [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-067-6 e-ISBN 978-1-61779-068-3 DOI 10.1007/978-1-61779-068-3 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011923966 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface Since its early beginnings of religious and “medicinal” purposes, blood science has become a cornerstone of medicine – especially, in the areas of clinical chemistry, disease diagnosis, and therapeutic monitoring. Because blood constituents, primarily proteins, reflect diverse physiological, pathological, and pharmacological states they are of great clinical significance – i.e., blood can be considered the “window of disease.” The ease with which blood (especially, its plasma/serum components) can be sampled in a noninvasive manner makes it a logical choice for diagnostic screening applications. Over the past decade, we have witnessed the advent of more powerful proteomics technologies that allow deeper drilling of the blood proteome. These technological improvements have, in part, fuelled the quest for the discovery of novel blood-based bi
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