Plant Signal Transduction Methods and Protocols

This fully updated volume reflects the spectacular advances in our knowledge of signal transduction pathways with a selection of ‘classic’ as well as newly developed approaches. These detailed approaches expand into the fields of molecular biology, bioche

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Jose R. Botella Miguel A. Botella Editors

Plant Signal Transduction Methods and Protocols Second Edition

METHODS

IN

MOLECULAR BIOLOGY

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Plant Signal Transduction Methods and Protocols Second Edition

Edited by

Jose R. Botella The University of Queensland, St. Lucia, QLD, Australia

Miguel A. Botella Universidad de Málaga, Málaga, Spain

Editors Jose R. Botella The University of Queensland St. Lucia, QLD, Australia

Miguel A. Botella Universidad de Málaga Málaga, Spain

ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3114-9 ISBN 978-1-4939-3115-6 (eBook) DOI 10.1007/978-1-4939-3115-6 Library of Congress Control Number: 2015952013 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)

Dedication Este libro lo queremos dedicar a nuestra madre, Pepita Mesa. Aunque siempre has estado presente en nuestras vidas, queremos que estas líneas sirvan para agradecerte toda tu dedicación y sacrificio, especialmente durante nuestros años de colegio e instituto. Siempre estabas ahí, encima nuestra, para asegurarte que hacíamos los deberes y estudiábamos cuando lo que queríamos era hacer el vago. Eras tú la que nos animaba cuando las cosas se torcían y nos apoyaba en los momentos difíciles. Con una madre así es mucho más fácil conseguir lo que te propones en la vida. Pero sobre todo, gracias por no rendirte nunca y tener una fe infinita en nosotros. Te queremos. Tus hijos Lale y Ramón. To our Mom. Thanks for a thousand sacrifices and for never giving up on us.

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Preface Plants need to perceive and acco