Redox-Mediated Signal Transduction Methods and Protocols
This volume explores the scope of the cellular redox analysis and the importance of not being limited by frequently changing and evolving technology. The chapters in this book cover a wide range of topics such as redox components in animal and plant cells
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John T. Hancock Myra E. Conway Editors
RedoxMediated Signal Transduction Methods and Protocols Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Redox-Mediated Signal Transduction Methods and Protocols Second Edition
Edited by
John T. Hancock and Myra E. Conway Department of Applied Sciences, University of the West of England, Bristol, UK
Editors John T. Hancock Department of Applied Sciences University of the West of England Bristol, UK
Myra E. Conway Department of Applied Sciences University of the West of England Bristol, UK
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9461-8 ISBN 978-1-4939-9463-2 (eBook) https://doi.org/10.1007/978-1-4939-9463-2 © Springer Science+Business Media, LLC, part of Springer Nature 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Cover Caption: Knockdown of the redox-active BCAT protein results in increased nuclear localization of MAPK in response to insulin. Image courtesy of: Mai Shafei, Arwa Flembon and David Corry. This Humana imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Cellular redox reactions are fundamentally important to bioenergetics, signalling, and overall homeostasis, yet their assessment, particularly in cells, can be challenging. Over the last decade, there has been a drive to develop more sophisticated, sensitive probes to better characterize redox modifications. This book is excellent for those who are not just new to the redox field but also for redox experts that aim to extend their research into new areas or new sys
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