Plant Vacuolar Trafficking Methods and Protocols
This volume provides a collection of state-of-the-art protocols on plant vacuolar trafficking. Chapters guide the reader through design and preparation of fusion proteins, expression vectors, and detailed analysis of different microscopy techniques. Writt
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Cláudia Pereira Editor
Plant Vacuolar Trafficking Methods and Protocols
Methods
in
M o l e c u l a r B i o lo g y
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Plant Vacuolar Trafficking Methods and Protocols
Edited by
Cláudia Pereira Faculty of Sciences, University of Porto, Porto, Portugal
Editor Cláudia Pereira Faculty of Sciences University of Porto Porto, Portugal
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7855-7 ISBN 978-1-4939-7856-4 (eBook) https://doi.org/10.1007/978-1-4939-7856-4 Library of Congress Control Number: 2018942329 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Vacuoles occupy a large volume in a plant cell and have important storage, homeostatic, and regulatory functions. Given the status of this organelle in the cell, it is crucial to understand not only its biogenesis but also how proteins reach and accumulate in the plant vacuole. Indeed, protein vacuolar trafficking is a major topic in the study of protein sorting in plants, being the issue of several studies for several years. This volume of the Methods in Molecular Biology series aims to provide the scientific community in this subject with a collection of a significant number of state-of-the-art protocols, ranging from the design and preparation of fusion proteins and expression vectors to a more detailed analysis involving different microscopy techniques. Plant Vacuol
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