T-Cell Trafficking Methods and Protocols
In the last decade, a large number of major discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses. In T-Cell Trafficking: Methods and Protocols, expert researchers explore how the development o
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MO L E C U L A R BI O L O G Y
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For other titles published in this series, go to www.springer.com/series/7651
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T-Cell Trafficking Methods and Protocols
Edited by
Federica M. Marelli-Berg Department of Immunology, Division of Medicine, Imperial College London, London, UK
Sussan Nourshargh William Harvey Research Institute, Barts and the London School of Medicine & Dentistry, Queen Mary University of London, London, UK
Editors Federica M. Marelli-Berg Department of Immunology Division of Medicine Imperial College London Hammersmith Hospital Du Cane Road London UK W12 0NN [email protected]
Sussan Nourshargh William Harvey Research Institute Barts and the London School of Medicine and Dentistry Queen Mary, University of London Charterhouse Square London UK EC1M 6BQ [email protected]
Additional material for this book can be downloaded from http://extras.springer.com ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-60761-460-9 e-ISBN 978-1-60761-461-6 DOI 10.1007/978-1-60761-461-6 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2010921222 © Springer Science+Business Media, LLC 2010 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface Both the development of a functional immune system and the establishment of effective immunity are orchestrated by a series of complex and coordinated migratory events. In the last decade a large number of major discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses, including the development of organized lymphoid tissue, the compartmentalized recirculation of lymphocyte subsets, and their targeted access to inflammatory antigenic sites. This has been made possible by the development and increasing availability of novel techniq
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