Promoting Effects on Watermelon and Fermentation Optimization of Plantibacter sp. WZW03
- PDF / 2,578,630 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 61 Downloads / 151 Views
Promoting Effects on Watermelon and Fermentation Optimization of Plantibacter sp. WZW03 Zhigang Wang1,2,3 · Yongjian Piao3 · Fugui Zhang3 · Yunlong Hu1 · Jin Zeng4 · Jun Nan2 Received: 4 April 2019 / Accepted: 1 October 2019 © Springer Science+Business Media, LLC, part of Springer Nature 2019
Abstract Plantibacter sp. WZW03 can effectively improve the utilization efficiency of soil potassium by plants. However, high production cost of Plantibacter sp. WZW03 has limited its utilization. The study assessed the plant growth-promoting ability of Plantibacter sp. WZW03 on watermelon (Citrullus lanatus L. cv. Jingxin No. 1) by pot experiment and investigated the fermentation process using different substrate by response surface analysis. The results showed Plantibacter sp. WZW03 exhibited the maximum indole-3-acetic acid (IAA) secretion of 89.41 mg/L at 96 h and siderophore activity of 30.68% at 48 h in Medium-K basalt medium. Application of Plantibacter sp. WZW03 to watermelon effectively promoted root growth and increased root weight, length, and volume. The optimal fermentation substrate of Plantibacter sp. WZW03 was 65.7% chicken manure, 32.9% straw, 0.9% starch, and 0.5% Fe(OH)3. The optimal conditions included the culture time on 20.36 h, culture temperature at 29.87 °C and C/N at 19:1. The maximum biomass was 1.47 × 109 cfu/mL based on the optimum conditions, in agreement with the predicted value of the response surface. Compared with the silicate bacteria medium, the culture time was shortened by 12 h, the biomass was improved by 95.52%, and the cost of the fermentation process was lowered by 89.04%. Plantibacter sp. WZW03 could promote root growth and optimize root structure in watermelon and present the strong capacity to secrete IAA and produce siderophores. The developed fermentation process significantly enhanced the bacterial biomass, shortened the fermentation period, and lowered the production cost, thus providing theoretical and technical support for applying Plantibacter sp. WZW03 to promote the growth of watermelon. Keywords Plantibacter sp. · Watermelon · Promoting effect · Medium optimization · Fermentation
Introduction Potassium is one of the essential macronutrients for crop growth (Liu et al. 2006; Hafsi et al. 2014), and it participates in each stage of plant growth and development (Zhao et al. * Jun Nan [email protected] 1
Department of Biotechnology, Institute of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 150000, China
2
Department of Environmental Science and Engineering, Institute of Municipal Environment and Engineering, Harbin Industry University, Harbin, China
3
Longjiang Environmental Protection Group Co., LTD, Harbin 150000, China
4
State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210000, China
2001). When a plant is exposed to environmental stress, potassium significantly enhances its capacity to resist the stress and can improve crop quality and yiel
Data Loading...
