Luteimonas weifangensis sp. nov., Isolated from Bensulfuron-Methyl Contaminated Watermelon Soil
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Luteimonas weifangensis sp. nov., Isolated from Bensulfuron‑Methyl Contaminated Watermelon Soil Hao Zhang1,2 · Hua‑Zhu Zhao1 · Juan Liu3 · Wei Qin3 · Xing Huang1 Received: 1 June 2020 / Accepted: 9 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract A Gram-stain-negative bacterium, designated WF-2T, was isolated from bensulfuron-methyl contaminated watermelon soil in Weifang, Shandong province, China. Cells of strain WF-2T were strictly aerobic, non-motile and rod-shaped. Strain WF-2T grew optimally at 30 °C, pH 7.0. Strain WF-2T possessed ubiquinone-8 (Q-8) as the predominant respiratory quinone. The major cellular fatty acids of the strain WF-2T (> 5.0%) were iso-C15:0, anteiso-C15:0, iso-C16:0, C16:0, iso-C17:0, and summed feature 9 (iso-C17:1 ω9c/C16:0 10-methyl). The polar lipids consisted of two unidentified lipids, three unidentified phospholipids, phosphatidylglycerol, phosphatidylethanolamine and diphosphatidyl glycolipid. Phylogenetic analysis based on 16S rRNA gTne sequences revealed that WF-2T was a member of the genus Luteimonas and showed the highest sequence similarity to Luteimonas lumbrici 1.1416T (98.6%) and Lysobacter pocheonensis Gsoil 193T (97.9%), lower sequence similarity ( 200 bp). The N50 of scaffolds was
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Fig. 1 Neighbor-joining phylogenetic tree shows the taxonomic position of strain WF-2T and other closely related species based on 16S rRNA gene sequences. Database accession numbers are given in parentheses following the organism names. Bootstrap values (expressed as percentages of 1000 replicates) greater than 50% are shown at branch points. Bar, 0.02 substitutions per nucleotide position
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Luteimonas mephitis DSM 12574T (AJ012228) Luteimonas soli Y2T (KP684142) Luteimonas notoginsengisoli SYP-B804T (KP076295) Luteimonas lutimaris G3T (GU199001)
Luteimonas weifangensis WF-2T (MH790417) Luteimonas lumbrici 1.1416T (ML636798)
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Luteimonas arsenica KCTC 42824T (KP208749) Luteimonas padinae CDR SL 15T (LN879436)
96
Luteimonas terricola BZ92rT (FJ948107) Luteimonas huabeiensis HB2T (JX658136)
99
Luteimonas marina FR1330T (EU295459) Luteimonas aestuarii B9T (EF660758) Luteimonas vadosa KMM9005T (AB704915) Lysobacter panaciterrae Gsoil 068T (AB245359) 84
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Luteimonas aquatica RIB1-20T (EF626688) Lysobacter ginsengisoli Gsoil 357T (AB245363) Lysobacter pocheonensis Gsoil193T (EU273938) Lysobacter lycopersici JCM 19164T (KC820654) Lysobacter sediminicola 7C-9T (JQ349048) Arenimonas caeni z29T (MG603321) Escherichia coli ATCC 11775T (X80725)
0.02
253,131 bp. The number of CDS in the genome is 2513. The number of genes relevant to carbohydrate metabolism, energy metabolism, amino acid metabolism, metabolism of cofactors and vitamins, gene expression, DNA binding was 110, 100, 131, 109, 108 and 100, respectively. The genomic DNA G + C content of strain WF-2T was 69.2 mol%. The DDH and ANI for the draft genomes between strain WF-2T and L. lumbrici 1.1416T were 24.7% and 81.9%. Both values are distinctly low
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