Affinity Chromatography Methods and Protocols
The aim of this edition is to introduce the beginner to the basics of affinity chromatography and provide practical knowledge for the development of affinity separation protocols. Affinity Chromatography: Methods and Protocols, Third Edition guides reader
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Senta Reichelt Editor
Affinity Chromatography Methods and Protocols Third Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
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Affinity Chromatography Methods and Protocols Third Edition
Edited by
Senta Reichelt Leibniz-Institut für Oberfl€achenmodifizierung, Leipzig, Germany
Editor Senta Reichelt Leibniz-Institut fu¨r Oberfl€achenmodifizierung Leipzig, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2446-2 ISBN 978-1-4939-2447-9 (eBook) DOI 10.1007/978-1-4939-2447-9 Library of Congress Control Number: 2015932256 Springer New York Heidelberg Dordrecht London # Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface The ongoing progress in biomedicine (e.g., identification of low abundant proteins in human blood) requires high standards of bioseparation and purification techniques. Affinity chromatography is one of the most selective and versatile types of liquid adsorption chromatography and is particularly suited for sophisticated purification processes. Notably, the concept of affinity chromatography is not a new state-of-the-art development. The first report on affinity chromatography-based separations was already published in the early 1900s. The Thomson Reuters database reveals more than 150,000 publications or reports including the term “affinity chromatography.” The number of publications is still increasing exponentially. The significance and timeliness of the topic are illustrated by the fact that more than 17,000 scientific articles, reports, or patents in English language were published since 2010 on this very field. D
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